{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T08:11:08Z","timestamp":1774512668106,"version":"3.50.1"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,12,28]],"date-time":"2025-12-28T00:00:00Z","timestamp":1766880000000},"content-version":"vor","delay-in-days":27,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical &amp;amp; Exp Dental Res"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>This study aimed to investigate the differentially expressed microRNAs in erosive oral lichen planus, followed by analyzing how the overexpression of identified miR\u2010516a\u20105p influences human oral mucosal fibroblasts.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Material and Methods<\/jats:title>\n                    <jats:p>High\u2010throughput sequencing using tissues from patients and healthy individuals identified varying microRNA expression profiles in erosive oral lichen planus tissues. Bioinformatics analysis subsequently revealed the enriched pathways and targeted genes involved. In vitro experiments were performed to confirm the validity of bioinformatic findings.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 82 microRNAs were differentially expressed in erosive oral lichen planus tissues. These microRNAs are mainly linked to T helper cell differentiation that enriched in MAPK signaling pathways, as well as targeting mRNAs like MAPK11. Overexpression of miR\u2010516a\u20105p resulted in a statistically significant decrease in MAPK11 mRNA level in human oral mucosal fibroblasts. Overexpression of miR\u2010516a\u20105p increased TNF\u2010\u03b1 and IFN\u2010\u03b3 levels, whereas it decreased IL\u20104, IL\u20106, IL\u201010, and IL\u201013 levels. Overexpression of miR\u2010516a\u20105p enhanced both the proliferation and migration of human oral mucosal fibroblasts.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The miR\u2010516a\u20105p overexpression in patients with erosive oral lichen planus may contribute to the imbalance of T helper 1\/2 cell\u2010associated inflammatory cytokine expression in human oral mucosal fibroblasts by targeting MAPK11 mRNA, promoting their proliferative and migratory capacities.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cre2.70270","type":"journal-article","created":{"date-parts":[[2025,12,29]],"date-time":"2025-12-29T06:04:17Z","timestamp":1766988257000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Overexpression of miR\u2010516a\u20105p Promotes Erosive Oral Lichen Planus: In Vitro Study Based on Bioinformatics Analyses"],"prefix":"10.1002","volume":"11","author":[{"given":"Yan","family":"Chen","sequence":"first","affiliation":[{"name":"Division of Pediatric Dentistry and Preventive Dentistry, School\/Hospital of Stomatology The First Affiliated Hospital of Xinjiang Medical University Urumqi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1443-4769","authenticated-orcid":false,"given":"Chen\u2011xi","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Oncology &amp; Surgery, School\/Hospital of Stomatology, The First Affiliated Hospital of Xinjiang Medical University National Clinical Medical Research Institute Urumqi China"},{"name":"Stomatological Research Institute of Xinjiang Uygur Autonomous Region Urumqi China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Xue","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine, 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