{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T18:27:46Z","timestamp":1791570466228,"version":"4.3.4"},"reference-count":77,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T00:00:00Z","timestamp":1767916800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T00:00:00Z","timestamp":1767916800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical &amp;amp; Exp Dental Res"],"published-print":{"date-parts":[[2026,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>The Janus kinase (JAK)\/Signal Transducer and Activator of Transcription (STAT) pathway plays a crucial role in oral inflammatory diseases such as oral lichen planus (OLP) and periodontitis. Interferon\u2010gamma (IFN\u2010\u03b3) is a cytokine that activates the JAK\/STAT pathway. This study aimed to evaluate the effect of the JAK1\/2 inhibitor ruxolitinib on IFN\u2010\u03b3\u2010driven inflammation in oral keratinocytes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Material and Methods<\/jats:title>\n                    <jats:p>Human oral keratinocytes (OKG4) were stimulated with 10\u2009ng\/mL IFN\u2010\u03b3 to assess the expression and phosphorylation status of JAK1, JAK2, STAT1, STAT2, and human leukocyte antigen (HLA)\u2010DRB1. Ruxolitinib (1\u2009\u00b5M) was used to inhibit IFN\u2010\u03b3\u2010induced signaling. Analyses were performed using Western blotting, immunofluorescence microscopy, and cell viability assays.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      IFN\u2010\u03b3 significantly upregulated pSTAT1 at 30\u2009min (control: 1.00\u2009\u00b1\u20090.00, IFN\u2010\u03b3: 29.17\u2009\u00b1\u20099.26,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0013), with sustained activation after 24\u2009h (IFN\u2010\u03b3: 29.49\u2009\u00b1\u200918.26,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0127). HLA\u2010DRB1 expression was also increased (control: 1.00\u2009\u00b1\u20090.00, IFN\u2010\u03b3: 3.51\u2009\u00b1\u20091.28,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0127). Co\u2010treatment with IFN\u2010\u03b3 and ruxolitinib reduced both pSTAT1 (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.3448) and HLA\u2010DRB1 (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.3448) expression to baseline levels. Ruxolitinib had no effect on cell viability (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&gt;\u20090.9999).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>IFN\u2010\u03b3 induces robust and sustained activation of STAT1 and upregulation of HLA\u2010DRB1 in oral keratinocytes, both effectively suppressed by ruxolitinib. These findings underscore the therapeutic potential of targeting the JAK\/STAT pathway in oral inflammatory diseases.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cre2.70281","type":"journal-article","created":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T07:31:55Z","timestamp":1768030315000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Ruxolitinib Suppresses Interferon\u2010\u03b3\u2010Induced JAK\/STAT Activation in Oral Keratinocytes"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0756-0310","authenticated-orcid":false,"given":"Kim N.","family":"Stolte","sequence":"first","affiliation":[{"name":"Department of Periodontology, Oral Medicine and Oral Surgery Charite\u0301 \u2010 Universita\u0308tsmedizin Berlin, corporate member of Freie Universit\u00e4t Berlin, Humboldt\u2010Universit\u00e4t zu Berlin, and Berlin Institute of Health Berlin Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Fedorova","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Oral Medicine and Oral Surgery Charite\u0301 \u2010 Universita\u0308tsmedizin Berlin, corporate member of Freie Universit\u00e4t Berlin, Humboldt\u2010Universit\u00e4t zu Berlin, and Berlin Institute of Health Berlin Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sameh","family":"Attia","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Oral Medicine and Oral Surgery Charite\u0301 \u2010 Universita\u0308tsmedizin Berlin, corporate member of Freie Universit\u00e4t Berlin, Humboldt\u2010Universit\u00e4t zu Berlin, and Berlin Institute of Health Berlin Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kerstin","family":"Danker","sequence":"additional","affiliation":[{"name":"Institute for Biochemistry Charite\u0301 \u2010 Universita\u0308tsmedizin Berlin, corporate member of Freie Universit\u00e4t Berlin, Humboldt\u2010Universit\u00e4t zu Berlin, and Berlin Institute of Health Berlin Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1043-2651","authenticated-orcid":false,"given":"Henrik","family":"Dommisch","sequence":"additional","affiliation":[{"name":"Department of Periodontology, Oral Medicine and Oral Surgery Charite\u0301 \u2010 Universita\u0308tsmedizin Berlin, corporate member of Freie Universit\u00e4t Berlin, Humboldt\u2010Universit\u00e4t zu Berlin, and Berlin Institute of Health Berlin Berlin 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