{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T13:45:13Z","timestamp":1782740713395,"version":"3.54.5"},"reference-count":15,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T00:00:00Z","timestamp":1779753600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T00:00:00Z","timestamp":1779753600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004479","name":"Natural Science Foundation of Jiangxi Province","doi-asserted-by":"publisher","award":["20232BAB206079"],"award-info":[{"award-number":["20232BAB206079"]}],"id":[{"id":"10.13039\/501100004479","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical &amp;amp; Exp Dental Res"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Orthodontic tooth movement (OTM) is frequently accompanied by inflammatory responses, including periodontitis, which may compromise treatment outcomes. This study investigated the protective role of quercetin, a natural flavonoid, in OTM\u2010associated periodontitis and its underlying mechanism involving macrophage metabolic reprogramming via the IL\u201017\/Hippo\/YAP axis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Material and Methods<\/jats:title>\n                    <jats:p>An in vivo OTM model was established using YAP\u2010wild\u2010type and YAP\u2010knockout Sprague\u2010Dawley rats to assess the effects of quercetin on periodontal inflammation and alveolar bone resorption. In vitro studies were conducted using the RAW264.7 macrophage cell line treated with quercetin (0.1\u201310\u2009\u03bcM). Key methodologies included micro\u2010CT analysis for alveolar bone loss, histopathological evaluation (H&amp;E and TRAP staining), qPCR and western blot analysis for gene and protein expression, ELISA for cytokine quantification, flow cytometry for apoptosis and cell cycle analysis, and CCK\u20108 assays for cell viability. The role of YAP was further validated using specific inhibitors and genetic knockout models.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Quercetin administration significantly attenuated periodontal inflammation and alveolar bone loss in the OTM model. In RAW264.7 macrophages, quercetin suppressed IL\u201017\u2010induced activation of the Hippo\/YAP pathway, inhibited YAP nuclear translocation, and downregulated the expression of key glycolytic enzymes (HK2, LDHA, and PKM2). Consequently, quercetin reduced lactate production and aerobic glycolysis. These effects were reversed upon YAP overexpression, confirming the pivotal role of the IL\u201017\/Hippo\/YAP axis in quercetin\u2010mediated metabolic modulation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Quercetin alleviates OTM\u2010associated periodontitis by inhibiting the IL\u201017\/Hippo\/YAP signaling pathway, thereby suppressing macrophage aerobic glycolysis. These findings highlight quercetin as a potential therapeutic adjunct in orthodontics to mitigate inflammatory complications.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cre2.70383","type":"journal-article","created":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T18:06:52Z","timestamp":1779818812000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Quercetin Inhibits Orthodontic Tooth Movement\u2010Associated Periodontitis by Regulating Macrophage Aerobic Glycolysis Through the IL\u201017\/Hippo\/YAP Axis"],"prefix":"10.1002","volume":"12","author":[{"given":"Fen","family":"Yao","sequence":"first","affiliation":[{"name":"Jiangxi Provincial Key Laboratory of Oral Diseases, Jiangxi Provincial Clinical Research Center for Oral Diseases The Affiliated Stomatological Hospital, Jiangxi MedicalCollege, Nanchang University Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiping","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Anesthesia, The First Affiliated Hospital, Jiangxi Medical College Nanchang University Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wuchao","family":"Wu","sequence":"additional","affiliation":[{"name":"Jiangxi Provincial Key Laboratory of Oral Diseases, Jiangxi Provincial Clinical Research Center for Oral Diseases The Affiliated Stomatological Hospital, Jiangxi MedicalCollege, Nanchang University Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bingqian","family":"Liu","sequence":"additional","affiliation":[{"name":"Jiangxi Provincial Key Laboratory of Oral Diseases, Jiangxi Provincial Clinical Research Center for Oral Diseases The Affiliated Stomatological Hospital, Jiangxi MedicalCollege, Nanchang University Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rongqi","family":"Xia","sequence":"additional","affiliation":[{"name":"Jiangxi Provincial Key Laboratory of Oral Diseases, Jiangxi Provincial Clinical Research Center for Oral Diseases The Affiliated Stomatological Hospital, Jiangxi MedicalCollege, Nanchang University Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao","family":"Zhou","sequence":"additional","affiliation":[{"name":"Jiangxi Provincial Key Laboratory of Oral Diseases, Jiangxi Provincial Clinical Research Center for Oral Diseases The Affiliated Stomatological Hospital, Jiangxi MedicalCollege, Nanchang University Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenxia","family":"Wang","sequence":"additional","affiliation":[{"name":"Jiangxi Provincial Key Laboratory of Oral Diseases, Jiangxi Provincial Clinical Research Center for Oral Diseases The Affiliated Stomatological Hospital, Jiangxi MedicalCollege, Nanchang University Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujie","family":"Chen","sequence":"additional","affiliation":[{"name":"Jiangxi Provincial Key Laboratory of Oral Diseases, Jiangxi Provincial Clinical Research Center for Oral Diseases The Affiliated Stomatological Hospital, Jiangxi MedicalCollege, Nanchang University Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengyu","family":"Liao","sequence":"additional","affiliation":[{"name":"Jiangxi Provincial Key Laboratory of Oral Diseases, Jiangxi Provincial Clinical Research Center for Oral Diseases The Affiliated Stomatological Hospital, Jiangxi MedicalCollege, Nanchang University Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,5,26]]},"reference":[{"key":"e_1_2_10_2_1","first-page":"64","article-title":"Effectiveness of Surgical and Non\u2010Surgical Techniques for Accelerating Orthodontic Tooth Movement in Fixed Appliances and Aligners: A Systematic Review","volume":"38","author":"Aline G.","year":"2025","journal-title":"Turkish Journal of Orthodontics"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.87057"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/cre2.70061"},{"key":"e_1_2_10_5_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0308619","article-title":"TAOK1\u2010Mediated Regulation of the YAP\/TEAD Pathway as a Potential Therapeutic Target in Heart Failure","volume":"19","author":"Jiani Z.","year":"2024","journal-title":"PLoS One"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.18350"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2025.04.011"},{"key":"e_1_2_10_8_1","first-page":"90","article-title":"Quercetin Mediates the Therapeutic Effect of Centella Asiatica on Psoriasis by Regulating STAT3 Phosphorylation to Inhibit the IL\u201023\/IL\u201017A Axis","volume":"45","author":"Qing L.","year":"2025","journal-title":"Nan fang yi ke da xue xue bao = Journal of Southern Medical University"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.14715\/cmb\/2024.70.6.33"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2025.1524814"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-025-87556-w"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00213-025-06784-0"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12903-024-05080-1"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2025.1526813"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2024.112933"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.apsb.2024.07.008"}],"container-title":["Clinical and Experimental 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