{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T13:45:06Z","timestamp":1782740706055,"version":"3.54.5"},"reference-count":45,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T00:00:00Z","timestamp":1781568000000},"content-version":"vor","delay-in-days":15,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical &amp;amp; Exp Dental Res"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>\n                      To evaluate serum IgG responses against\n                      <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n                      gingipains RgpB and Kgp in patients with stage III and IV periodontitis and examine associations with clinical periodontal parameters.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>This exploratory cross\u2010sectional study included participants with advanced periodontitis (Stage III and IV). Clinical periodontal parameters (number of teeth, bleeding on probing in percent [BoP%], probing pocket depth [PPD], bone loss\u2010to\u2010age ratio, periodontal inflamed surface area [PISA] per tooth) were recorded. Blood samples were collected and serum IgG responses against recombinant RgpB and Kgp were analyzed. Serum IgG signals were quantified using enzyme\u2010linked immunosorbent assay (ELISA).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      All 42 participants (mean age 62 years) exhibited measurable serum IgG responses against\n                      <jats:italic>P. gingivalis<\/jats:italic>\n                      gingipains. IgG signals (optical density) ranged from 0.13 to 1.46 for RgpB and 0.34 to 1.51 for Kgp. RgpB\u2010specific IgG levels were higher in Stage IV versus Stage III periodontitis (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.023), whereas Kgp\u2010specific IgG levels showed no significant difference between stages (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.249). Anti\u2010RgpB IgG levels correlated positively with age (\n                      <jats:italic>\u03c1<\/jats:italic>\n                      \u2009=\u20090.362,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.019) and inversely with the number of teeth (\n                      <jats:italic>\u03c1<\/jats:italic>\n                      \u2009=\u2009\u22120.372,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.015), whereas anti\u2010Kgp IgG showed no statistically significant correlations with the assessed clinical parameters.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Serum IgG binding signals to RgpB and Kgp were measurable by ELISA and showed substantial heterogeneity among patients with advanced periodontitis, with considerable overlap between disease stages. Anti\u2010RgpB IgG was associated with age and tooth loss, whereas inflammatory measures showed no significant correlations and anti\u2010Kgp IgG showed only weak trend\u2010level associations. Overall, total gingipain\u2010specific IgG appears to reflect cumulative periodontal disease burden and exposure history more clearly than current inflammatory activity in this cohort, with limited value as a standalone marker for differentiating Stage III and Stage IV periodontitis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cre2.70391","type":"journal-article","created":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T17:34:17Z","timestamp":1781631257000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Heterogeneous Systemic IgG Responses to\n                    <i>Porphyromonas gingivalis<\/i>\n                    Gingipains in Advanced Periodontitis"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0402-5312","authenticated-orcid":false,"given":"Steffen","family":"B\u00fcttner","sequence":"first","affiliation":[{"name":"Faculty of Medicine and University Hospital Cologne, Polyclinic for Operative Dentistry and Periodontology University of Cologne Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2239-6010","authenticated-orcid":false,"given":"Sonja H. M.","family":"Derman","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and University Hospital Cologne, Polyclinic for Operative Dentistry and Periodontology University of Cologne Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2945-9897","authenticated-orcid":false,"given":"Alexander","family":"Simonis","sequence":"additional","affiliation":[{"name":"Department I of Internal Medicine Faculty of Medicine and University Hospital Cologne University of Cologne Cologne Germany"},{"name":"Faculty of Medicine and University Hospital Cologne Center for Molecular Medicine Cologne University of Cologne Cologne Germany"},{"name":"German Center for Infection Research (DZIF), Partner Site Bonn\u2010Cologne Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3296-7553","authenticated-orcid":false,"given":"Alicia M.","family":"Meyer\u2010Hofmann","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and University Hospital Cologne, Polyclinic for Operative Dentistry and Periodontology University of Cologne Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7390-1557","authenticated-orcid":false,"given":"Max","family":"von Kohout","sequence":"additional","affiliation":[{"name":"Institute for Medical Statistics and Computational Biology University of Cologne Cologne Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2962-7814","authenticated-orcid":false,"given":"Manuel","family":"Koch","sequence":"additional","affiliation":[{"name":"Faculty of Medicine and University Hospital Cologne Institute for Dental Research and Oral Musculoskeletal Biology University of Cologne Cologne Germany"},{"name":"Faculty of Medicine and University Hospital Cologne Center for Dental, Oral 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