{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T13:44:33Z","timestamp":1792071873603,"version":"4.4.0"},"reference-count":41,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T00:00:00Z","timestamp":1787097600000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical &amp; Exp Dental Res"],"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Electrospun nanofibrous scaffolds that mimic extracellular matrix (ECM) architecture are promising for endodontic and craniofacial tissue engineering. This study aimed to fabricate a composite electrospun scaffold comprising poly(\u03b5\u2010caprolactone) (PCL), collagen, mineral trioxide aggregate (MTA), and a TAT cell\u2010penetrating peptide, and to evaluate its physicochemical properties and short\u2010term cytocompatibility with fibroblasts.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Material and Methods<\/jats:title>\n                    <jats:p>Electrospun mats were prepared from PCL and PCL\u2013collagen blends with or without incorporation of MTA and TAT peptide. Fiber morphology and diameter were assessed by scanning electron microscopy (SEM). Chemical structure was analyzed using Fourier\u2010transform infrared (FTIR) spectroscopy. Surface charge was determined by zeta potential measurement before and after TAT functionalization. Tensile properties of the PCL\u2013collagen\u2013MTA\u2013TAT scaffold were evaluated using uniaxial testing. Cytotoxicity toward human fibroblasts was assessed using the MTT assay after 24 and 48\u2009h of culture on PCL, PCL\u2013collagen, PCL\u2013collagen\u2013MTA, and PCL\u2013collagen\u2013MTA\u2013TAT scaffolds. Additional physicochemical characterization included water contact angle and biodegradability.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>SEM revealed continuous, bead\u2010free fibers forming a porous, interconnected network with submicron diameters in all groups. FTIR spectra confirmed the presence of the expected components in the composite scaffold. Zeta potential shifted from negative values in PCL\u2013collagen\u2013MTA scaffolds to positive values after TAT incorporation, indicating surface modification. The PCL\u2013collagen\u2013MTA\u2013TAT scaffold exhibited tensile behavior compatible with handling. MTT results showed fibroblast viability exceeding 90% for all scaffold compositions at both time points relative to tissue culture plastic controls, with no evidence of cytotoxicity. Water contact angle and biodegradability suggested that incorporation of collagen, MTA, and TAT improved scaffold surface characteristics.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The electrospun PCL\u2013collagen\u2013MTA\u2013TAT scaffold demonstrated ECM\u2010like morphology, modified surface charge, acceptable handling properties, and short\u2010term fibroblast compatibility. This composite nanofibrous system may serve as a preliminary platform for further investigation in bone and dental tissue engineering; however, additional functional and long\u2010term biological studies are required to confirm its regenerative potential.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cre2.70437","type":"journal-article","created":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T06:42:27Z","timestamp":1787208147000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Fabrication and Characterization of a Trans\u2010Activator of Transcription Peptide\u2013Functionalized Collagen\u2013Mineral Trioxide Aggregate Nanofiber Scaffold: Physicochemical Properties and Fibroblast Response"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0458-9436","authenticated-orcid":false,"given":"Mehdi","family":"Sheikharabi","sequence":"first","affiliation":[{"name":"Department of Medical Biotechnology, Faculty of Advanced Technologies Golestan University of Medical Sciences Gorgan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-9231-5212","authenticated-orcid":false,"given":"Javad","family":"Delkabadi","sequence":"additional","affiliation":[{"name":"Department of Radiology, Faculty of Dentistry Mashhad University of Medical Sciences Mashhad Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2340-1870","authenticated-orcid":false,"given":"Ayyoob","family":"Khosravi","sequence":"additional","affiliation":[{"name":"Stem Cell Research Center, Biomedical Research Institute Golestan University of Medical Sciences Gorgan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0557-767X","authenticated-orcid":false,"given":"Ezatolah","family":"Kazeminejad","sequence":"additional","affiliation":[{"name":"Dental Research Center, Jorjani Clinical Sciences Research Institute Golestan University of Medical Sciences Gorgan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,19]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3109\/21691401.2016.1146731"},{"issue":"14","key":"e_1_2_11_3_1","first-page":"1","article-title":"Electrospun Polycaprolactone Nanofibers: Current Research and Applications in Tissue Engineering","volume":"13","author":"Azari A.","year":"2021","journal-title":"Polymers (Basel)"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/26415275.2020.1848432"},{"issue":"8","key":"e_1_2_11_5_1","article-title":"Polycaprolactone Usage in Additive Manufacturing Strategies for Tissue Engineering Applications: A Review","volume":"110","author":"Backes E. 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