{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:46:19Z","timestamp":1753868779942,"version":"3.41.2"},"reference-count":52,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,2,6]],"date-time":"2024-02-06T00:00:00Z","timestamp":1707177600000},"content-version":"vor","delay-in-days":5,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical &amp;amp; Exp Dental Res"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>The aim of this study was the assessment of the efficiency of the ethyl acetate (EthOAc) extract of <jats:italic>Thymus serpyllum<\/jats:italic> against <jats:italic>Candida albicans<\/jats:italic> and to compare it with sodium hypochlorite (NaOCl) and chlorhexidine (CHX), as well as their genotoxic effect.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>The antifungal effectiveness of the EthOAc extract of <jats:italic>Thymus serpyllum<\/jats:italic> was determined using the agar disk diffusion method. The inhibition zones induced by the EthOAc extract were compared after 5\u2009min, 60\u2009min, and 24\u2009h to those induced by standard solutions (2% CHX and 2% NaOCl). An in vitro genotoxicity assay was performed in cultured lymphocytes from the blood of human volunteers to observe micronuclei formation. Statistical analysis of the results was performed using the Kruskal\u2013Wallis test and one\u2010way analysis of variance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The inhibition zone of combination of CHX with EthOAc extract of <jats:italic>Thymus serpyllum<\/jats:italic> against <jats:italic>C. albicans<\/jats:italic> was 29.7\u2009mm after 5\u2009min, 28.3\u2009mm after 60\u2009min, and 29\u2009mm after 24\u2009h. The inhibition zone of NaOCl in combination with EthOAc extract of <jats:italic>Thymus serpyllum<\/jats:italic> against <jats:italic>C. albicans<\/jats:italic> was 0\u2009mm. The EthOAc extract of <jats:italic>Thymus serpyllum<\/jats:italic> did not show a genotoxic effect on lymphocyte cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The EthOAc extract of <jats:italic>Thymus serpyllum<\/jats:italic> in combination with CHX may be a useful root canal disinfection in endodontic therapy.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cre2.837","type":"journal-article","created":{"date-parts":[[2024,2,7]],"date-time":"2024-02-07T06:41:01Z","timestamp":1707288061000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Antifungal and genotoxic effects of <i>Thymus serpyllum<\/i> as a root canal irrigant"],"prefix":"10.1002","volume":"10","author":[{"given":"Ariana","family":"Kameri","sequence":"first","affiliation":[{"name":"Department of Dental Pathology and Endodontics, Faculty of Medicine University of Prishtina \u201cHasan Prishtina\u201d  Prishtina Kosova"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9738-3671","authenticated-orcid":false,"given":"Agime","family":"Dragidella","sequence":"additional","affiliation":[{"name":"Department of Dental Pathology and Endodontics, Faculty of Medicine University of Prishtina \u201cHasan Prishtina\u201d  Prishtina Kosova"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arben","family":"Haziri","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Natural Science Faculty University of Prishtina \u201cHasan Prishtina\u201d  Prishtina Kosova"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zeqir","family":"Hashani","sequence":"additional","affiliation":[{"name":"Faculty of Education University \u201cFehmi Agani\u201d  Gjakove Kosova"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kemajl","family":"Kurteshi","sequence":"additional","affiliation":[{"name":"Department of Biology, Natural Science Faculty University of Prishtina \u201cHasan Prishtina\u201d  Prishtina Kosova"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arsim","family":"Kurti","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Medical Faculty University of Prishtina \u201cHasan Prishtina\u201d  Prishtina Kosova"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,2,6]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1177\/1934578X1400900442"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/app11114759"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-92050-0_9"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1834-7819.2007.tb00527.x"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpha.2015.11.005"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules27248999"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2019.00587"},{"key":"e_1_2_11_9_1","first-page":"257","article-title":"Smear layer removal from root canal dentin and antimicrobial effect of citric acid\u2010modified chlorhexidine","volume":"5","author":"Dewi A.","year":"2020","journal-title":"European Endodontic Journal"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/2156587217700772"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(93)90049-L"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0027-5107(86)90010-2"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdent.2019.05.019"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-018-0298-x"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13238-018-0548-1"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1590\/0103-6440201302188"},{"key":"e_1_2_11_17_1","first-page":"1","article-title":"Kirby\u2010Bauer disk diffusion susceptibility test protocol","author":"Hudzicki J.","year":"2016","journal-title":"American Society for Microbiology"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/ijos.2013.12"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.9790\/3008-09260610"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1080\/10412905.2021.1955761"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbdv.201200041"},{"key":"e_1_2_11_22_1","first-page":"1152","volume-title":"Manual of clinical microbiology","author":"Jorgensen J. 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