{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T02:01:15Z","timestamp":1790215275876,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,7,18]],"date-time":"2024-07-18T00:00:00Z","timestamp":1721260800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical &amp;amp; Exp Dental Res"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Reflecting the need for an effective support for the daily oral hygiene routine of patients experiencing (symptoms of) gum inflammation, a new mouthwash has been developed containing an amine\u2009+\u2009zinc lactate\u2009+\u2009fluoride system. The in vitro efficacy of this product was assessed using traditional laboratory methods, as well as novel experimentation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>This mouthwash has been evaluated in a series of laboratory tests including two short interval kill tests (SIKTs), a 12\u2010h (longer term) biofilm regrowth assay, a plaque glycolysis assay, and an aerobic, repeated exposure biofilm model, as well as tests for soft tissue uptake and LPS neutralization.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Several laboratory studies demonstrate that a mouthwash containing an amine\u2009+\u2009zinc lactate\u2009+\u2009fluoride system provides short\u2010term and long\u2010term antibacterial activity. While the immediate efficacy of this formula has been shown to be driven by the presence of the amine, zinc lactate provides a long\u2010term antibacterial effect, as well as is able to inhibit bacterial metabolism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This research provides the basis for understanding the mode of action of this new mouthwash formulation and explains the previously observed clinical efficacy of this formula against plaque and gingivitis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cre2.874","type":"journal-article","created":{"date-parts":[[2024,7,18]],"date-time":"2024-07-18T12:14:45Z","timestamp":1721304885000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Antibacterial and oral tissue effectiveness of a mouthwash with a novel active system of amine\u2009+\u2009zinc lactate\u2009+\u2009fluoride"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-6038-0634","authenticated-orcid":false,"given":"Lyndsay M.","family":"Schaeffer","sequence":"first","affiliation":[{"name":"Colgate\u2010Palmolive Technology Center Piscataway New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-2671-5095","authenticated-orcid":false,"given":"Ying","family":"Yang","sequence":"additional","affiliation":[{"name":"Colgate\u2010Palmolive Technology Center Piscataway New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2918-5489","authenticated-orcid":false,"given":"Carlo","family":"Daep","sequence":"additional","affiliation":[{"name":"Colgate\u2010Palmolive Technology Center Piscataway New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-3280-7202","authenticated-orcid":false,"given":"Ekta","family":"Makwana","sequence":"additional","affiliation":[{"name":"Colgate\u2010Palmolive Technology Center Piscataway New Jersey USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3729-794X","authenticated-orcid":false,"given":"Golnaz","family":"Isapour","sequence":"additional","affiliation":[{"name":"Colgate\u2010Palmolive Europe S\u00e0rl Therwil Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-3797-270X","authenticated-orcid":false,"given":"Norbert","family":"Huber","sequence":"additional","affiliation":[{"name":"Colgate\u2010Palmolive Europe S\u00e0rl Therwil Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,7,18]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6831-12-1"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1590\/1678-775720160178"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/JPER.17-0719"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2004.00578.x"},{"key":"e_1_2_9_6_1","first-page":"33","article-title":"A clinical investigation of a dual zinc plus arginine dentifrice in reducing established dental plaque and gingivitis over a six\u2010month period of product use","volume":"29","author":"Delgado E.","year":"2018","journal-title":"The Journal of Clinical Dentistry"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12903-017-0471-5"},{"issue":"1","key":"e_1_2_9_8_1","first-page":"9","article-title":"Extrinsic dental stain caused by stannous fluoride","volume":"90","author":"Ellingsen J. 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