{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T16:48:04Z","timestamp":1792514884199,"version":"4.5.0"},"reference-count":42,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,6,9]],"date-time":"2024-06-09T00:00:00Z","timestamp":1717891200000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical &amp;amp; Exp Dental Res"],"published-print":{"date-parts":[[2024,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>The objective of this study was to investigate the effectiveness of testing for active matrix metalloproteinase\u20108 (aMMP\u20108) by a quantitative point\u2010of\u2010care (PoC), chairside lateral flow immunotest and azurocidin, in the peri\u2010implant sulcular fluid (PISF), as biomarkers for the presence or absence of peri\u2010implant diseases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Background<\/jats:title><jats:p>Current research indicates that proinflammatory cytokines and extracellular matrix\u2010degrading enzymes may be of value to diagnose and predict peri\u2010implant disease initiation and progression, but more data are needed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Eighty patients with implants were recruited. PISF samples were collected and quantitatively analyzed for aMMP\u20108 (chairside) and azurocidin with ELISA. Radiographic assessments and clinical indices (probing depth, probing attachment level, bleeding on probing, and plaque) were recorded after sampling. Kruskal\u2010Wallis test and pairwise post hoc Dunn\u2010Bonferroni test were used to relate aMMP\u20108 levels and azurocidin levels to clinical parameters. The diagnostic ability of aMMP\u20108 (ng\/mL) and azurocidin was analyzed by receiver operator curve analysis. Area under the curve (AUC) was calculated and the Spearman's rho, and the coefficient of determination (R<jats:sup>2<\/jats:sup>) were used to calculate the correlations between aMMP\u20108, azurocidin, and periodontal parameters.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Statistically significant differences were observed for aMMP\u20108 levels but not for azurocidin between healthy implants, implants with mucositis, and those with peri\u2010implantitis (13.65\u2009\u00b1\u20097.18, 32.33\u2009\u00b1\u200921.20, and 73.07\u2009\u00b1\u200943.93\u2009ng\/mL, respectively), (Kruskall\u2013Wallis test <jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). The aMMP\u20108 test with a threshold of 20\u2009ng\/mL has a sensitivity of 71.7% and a specificity of 77.8% to identify peri\u2010implantitis and healthy implants, respectively. AUC was found to be 0.814, and the accuracy of the method reaches 73.8%. Above a cutoff value of 33.7\u2009ng\/mL of aMMP\u20108, the accuracy of the test to detect peri\u2010implantitis\u00a0reaches 77.5% in relation to 62.5% of BoP from the same site.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Taken collectively, present data indicate that the aMMP\u20108 PoC lateral flow immunotest can be a beneficial, adjunctive diagnostic quantitative tool for real\u2010time screening for peri\u2010implant diseases.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cre2.883","type":"journal-article","created":{"date-parts":[[2024,6,10]],"date-time":"2024-06-10T05:53:12Z","timestamp":1717998792000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Diagnostic value of aMMP\u20108 and azurocidin in peri\u2010implant sulcular fluid as biomarkers of peri\u2010implant health or disease"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3136-706X","authenticated-orcid":false,"given":"Vithleem","family":"Xanthopoulou","sequence":"first","affiliation":[{"name":"Department of Preventive Dentistry, Periodontology and Implant Biology, Dental School Aristotle University of Thessaloniki Thessaloniki Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5821-5299","authenticated-orcid":false,"given":"Ismo T.","family":"R\u00e4is\u00e4nen","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Diseases, Head and Neck Center University of Helsinki and Helsinki University Hospital Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timo","family":"Sorsa","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Diseases, Head and Neck Center University of Helsinki and Helsinki University Hospital Helsinki Finland"},{"name":"Division of Periodontology, Department of Dental Medicine Karolinska Institutet Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8801-9184","authenticated-orcid":false,"given":"Dimitrios","family":"Tortopidis","sequence":"additional","affiliation":[{"name":"Department of Prosthodontics, Dental School Aristotle University of Thessaloniki Thessaloniki Greece"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitra","family":"Sakellari","sequence":"additional","affiliation":[{"name":"Department of Preventive Dentistry, Periodontology and Implant Biology, Dental School Aristotle University of Thessaloniki Thessaloniki 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