{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T05:07:54Z","timestamp":1772082474147,"version":"3.50.1"},"reference-count":47,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,7,16]],"date-time":"2024-07-16T00:00:00Z","timestamp":1721088000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004482","name":"Kuwait University","doi-asserted-by":"publisher","award":["DS02\/20"],"award-info":[{"award-number":["DS02\/20"]}],"id":[{"id":"10.13039\/501100004482","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical &amp;amp; Exp Dental Res"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Guided bone regeneration (GBR) is a core procedure used to regenerate bone defects. The aim of the study was to investigate the adherence of <jats:italic>Candida albicans<\/jats:italic> on six commercially available polytetrafluoroethylene (PTFE) membranes used in GBR procedures and the subsequent clinical consequences.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Six commercially available PTFE membranes were tested. Two of the membranes had a textured surface and the other four a plane, nontextured one. <jats:italic>C. albicans<\/jats:italic> (ATCC 24433) was cultured for 24\u2009h, and its cell surface hydrophobicity was assessed using a modified method. <jats:italic>C. albicans<\/jats:italic> adhesion to membrane discs was studied by scanning electron microscopy (SEM) and real\u2010time\u00a0polymerase chain reaction (PCR).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>C. albicans<\/jats:italic> was found to be hydrophobic (77.25%). SEM analysis showed that <jats:italic>C. albicans<\/jats:italic> adherence to all membranes examined was characterized by patchy, scattered, and small clustered patterns except for one nontextured membrane with a most rough surface in which a thick biofilm was observed. Real\u2010time\u00a0PCR quantification revealed significantly greater adhesion of <jats:italic>C. albicans<\/jats:italic> cells to PTFE membranes than the control membrane (<jats:italic>p<\/jats:italic>\u2009\u2264\u2009.001) with the membranes having a textured surface exhibiting the highest\u00a0count of 2680\u2009\u00d7\u200910<jats:sup>4<\/jats:sup> cells\/ml compared to the count of 707\u2009\u00d7\u200910<jats:sup>4<\/jats:sup>\u2009cells\/mL on those with a nontextured one (<jats:italic>p<\/jats:italic>\u2009\u2264\u2009.001). One membrane with nontextured surface, but with most rough surface was found to exhibit the highest count of 3010\u2009\u00d7\u200910<jats:sup>4<\/jats:sup> cells\/ml (<jats:italic>p<\/jats:italic>\u2009\u2264\u2009.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The results of this study indicate that <jats:italic>C. albicans<\/jats:italic> adhesion on membranes' surfaces depends on the degree of surface roughness and\/or on the presence of a texture. Textured PTFE membranes and\/or membranes high roughness showed significantly more adhered <jats:italic>C. albicans<\/jats:italic> cells. These findings can impact the surgeon's choice of GBR membrane and postoperative maintenance.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cre2.902","type":"journal-article","created":{"date-parts":[[2024,7,17]],"date-time":"2024-07-17T04:40:40Z","timestamp":1721191240000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Adhesion of <i>Candida albicans<\/i>\u00a0on PTFE membranes used in guided bone regeneration"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7797-5816","authenticated-orcid":false,"given":"Adel","family":"Al\u2010Asfour","sequence":"first","affiliation":[{"name":"Department of Surgical Sciences, College of Dentistry Kuwait University  Safat 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