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The enzyme stereoselectively reduces several ketones in a sustainable substrate\u2010coupled approach using 2\u2010propanol (5\u2009% v\/v) as hydrogen donor. The enzyme maintained its activity in organic co\u2010solvents in biphasic as well as monophasic systems and was even active in micro\u2010aqueous media (1\u2009% v\/v aqueous buffer). In general, a higher conversion was observed at higher log\u2009P values of the solvent, however, DMSO, which exhibits the lowest log\u2009P value of all solvents investigated, was not only tolerated but led to a higher conversion and relative activity (110\u2013210\u2009%). For example, the conversion after 24\u2005h in 15\u2009% v\/v DMSO was double that for the reaction performed in buffer. 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