{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:42:57Z","timestamp":1762101777864,"version":"build-2065373602"},"reference-count":26,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2015,10,23]],"date-time":"2015-10-23T00:00:00Z","timestamp":1445558400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Swedish research Council"},{"name":"Kempe foundation"},{"name":"The Knut and Alice Wallenberg Foundation"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemSusChem"],"published-print":{"date-parts":[[2015,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The integration of highly efficient enzymatic catalysis with the solvation properties of ionic liquids for an environmentally friendly and efficient use of raw materials such as wood requires fundamental knowledge about the influence of relevant ionic liquids on enzymes. Switchable ionic liquids (SIL) are promising candidates for implementation of enzymatic treatments of raw materials. One industrially interesting SIL is constituted by monoethanol amine (MEA) and 1,8\u2010diazabicyclo\u2010[5.4.0]\u2010undec\u20107\u2010ene (DBU) formed with sulfur dioxide (SO<jats:sub>2<\/jats:sub>) as the coupling media (DBU\u2010SO<jats:sub>2<\/jats:sub>\u2010MEASIL). It has the ability to solubilize the matrix of lignocellulosic biomass while leaving the cellulose backbone intact. Using a novel <jats:sup>31<\/jats:sup>P\u2005NMR\u2010based real\u2010time assay we show that this SIL is compatible with enzymatic catalysis because a model enzyme, adenylate kinase, retains its activity in up to at least 25\u2005wt\u2009% of DBU\u2010SO<jats:sub>2<\/jats:sub>\u2010MEASIL. Thus this SIL appears suitable for, for example, enzymatic degradation of hemicellulose.<\/jats:p>","DOI":"10.1002\/cssc.201501104","type":"journal-article","created":{"date-parts":[[2015,10,23]],"date-time":"2015-10-23T06:27:28Z","timestamp":1445581648000},"page":"3764-3768","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Realtime <sup>31<\/sup>P\u2005NMR Investigation on the Catalytic Behavior of the Enzyme Adenylate kinase in the Matrix of a Switchable Ionic Liquid"],"prefix":"10.1002","volume":"8","author":[{"given":"Per","family":"Rogne","sequence":"first","affiliation":[{"name":"Department of Chemistry Ume\u00e5 University  Linnaeus v\u00e4g 10 901 87 Ume\u00e5 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tobias","family":"Sparrman","sequence":"additional","affiliation":[{"name":"Department of Chemistry Ume\u00e5 University  Linnaeus v\u00e4g 10 901 87 Ume\u00e5 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ikenna","family":"Anugwom","sequence":"additional","affiliation":[{"name":"Department of Chemistry Ume\u00e5 University  Linnaeus v\u00e4g 10 901 87 Ume\u00e5 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jyri\u2010Pekka","family":"Mikkola","sequence":"additional","affiliation":[{"name":"Department of Chemistry Ume\u00e5 University  Linnaeus v\u00e4g 10 901 87 Ume\u00e5 Sweden"},{"name":"Laboratory of Industrial Chemistry and Reaction Engineering Johan Gadolin Process Chemistry Centre \u00c5bo Akademi University  \u00c5bo-Turku 20500 Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magnus","family":"Wolf\u2010Watz","sequence":"additional","affiliation":[{"name":"Department of Chemistry Ume\u00e5 University  Linnaeus v\u00e4g 10 901 87 Ume\u00e5 Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,10,23]]},"reference":[{"volume-title":"Ionic Liquids in Synthesis","year":"2006","key":"e_1_2_2_1_1"},{"key":"e_1_2_2_2_1","unstructured":"\u00a0"},{"key":"e_1_2_2_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/cssc.201300773"},{"key":"e_1_2_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biombioe.2014.08.005"},{"key":"e_1_2_2_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr050946x"},{"key":"e_1_2_2_6_1","unstructured":"\u00a0"},{"key":"e_1_2_2_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature06407"},{"key":"e_1_2_2_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature06410"},{"key":"e_1_2_2_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms1106"},{"key":"e_1_2_2_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja3032482"},{"key":"e_1_2_2_11_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja075055g"},{"key":"e_1_2_2_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb821"},{"key":"e_1_2_2_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/0263-7855(96)00009-4"},{"key":"e_1_2_2_14_1","unstructured":"\u00a0"},{"key":"e_1_2_2_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.72.12.4696"},{"key":"e_1_2_2_16_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00449a003"},{"key":"e_1_2_2_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr050312q"},{"key":"e_1_2_2_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)92037-5"},{"key":"e_1_2_2_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(20)81346-5"},{"key":"e_1_2_2_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi301460k"},{"key":"e_1_2_2_21_1","doi-asserted-by":"publisher","DOI":"10.1039\/c1ra00154j"},{"key":"e_1_2_2_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00413a020"},{"key":"e_1_2_2_23_1","unstructured":"\u00a0"},{"key":"e_1_2_2_24_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja051306e"},{"key":"e_1_2_2_25_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10858-005-4425-x"},{"key":"e_1_2_2_26_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmre.2000.2022"}],"container-title":["ChemSusChem"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcssc.201501104","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cssc.201501104","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,13]],"date-time":"2025-10-13T09:56:23Z","timestamp":1760349383000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/cssc.201501104"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,10,23]]},"references-count":26,"journal-issue":{"issue":"22","published-print":{"date-parts":[[2015,11]]}},"alternative-id":["10.1002\/cssc.201501104"],"URL":"https:\/\/doi.org\/10.1002\/cssc.201501104","archive":["Portico"],"relation":{},"ISSN":["1864-5631","1864-564X"],"issn-type":[{"type":"print","value":"1864-5631"},{"type":"electronic","value":"1864-564X"}],"subject":[],"published":{"date-parts":[[2015,10,23]]},"assertion":[{"value":"2015-08-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-10-23","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}