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In this study, an enzyme\u2010rich hydrogel monolithic microreactor was developed via in\u2005situ microfluidic assembly of inexpensive crude enzymes. This porous gel biocatalyst exhibited good tethering functions to nicotinamide cofactors; thus, they were retained by the hydrogel to controllably form a novel heterogeneous biocatalyst with self\u2010sufficient cofactors. The flow asymmetric production of a chiral alcohol in this cofactor\u2010entrapped gel microreactor achieved &gt;99\u2009% enantioselectivity and a high space\u2010time yield of 46.3\u2005g\u2009L\n                    <jats:sup>\u22121<\/jats:sup>\n                    \u2009h\n                    <jats:sup>\u22121<\/jats:sup>\n                    at 94.8\u2009% conversion. Moreover, the turnover number of cofactors reached as high as 4800 after continuous operation of 160 reactor volumes, realizing significantly higher utilization of the cofactors compared with many reported strategies. Furthermore, this engineered heterogeneous biocatalyst exhibited improved performance in terms of product tolerance and storage stability, paving the way for a green, cost\u2010effective, and sustainable continuous\u2010flow production of enantiopure alcohols.\n                  <\/jats:p>","DOI":"10.1002\/cssc.202201654","type":"journal-article","created":{"date-parts":[[2022,10,21]],"date-time":"2022-10-21T08:47:59Z","timestamp":1666342079000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Recycling of Cofactors in Crude Enzyme Hydrogels as Co\u2010immobilized Heterogeneous Biocatalysts for Continuous\u2010Flow Asymmetric Reduction of Ketones"],"prefix":"10.1002","volume":"16","author":[{"given":"Qiang","family":"Chen","sequence":"first","affiliation":[{"name":"The State Key Lab of Chemical Engineering Department of Chemical Engineering Tsinghua University  Beijing 100084 P. R. 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