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In this study, we communicate a convenient one\u2010pot synthesis of N,N\u2010dimethyl chitosan oligosaccharide (DMCOS) from chitin via acid\u2010catalyzed tandem depolymerization\u2010deacetylation\u2010N\u2010methylation pathway using formaldehyde as the methylation reagent. The synthesis protocol offers 77\u2009% DMCOS that features a high degree of deacetylation, a high degree of methylation, and a low average molecular weight. Compared to chitosan, DMCOS exhibits superior antifungal activity against <jats:italic>Candida<\/jats:italic> species. Mechanism study reveals a previously non\u2010reported hydroxyl group\u2010assisted effect that facilitates the reductive amination reaction under strong acidic conditions. Overall, our findings demonstrate the feasibility of direct synthesis of DMCOS from chitin, highlighting its potential use in anti\u2010fungal applications.<\/jats:p>","DOI":"10.1002\/cssc.202300591","type":"journal-article","created":{"date-parts":[[2023,6,19]],"date-time":"2023-06-19T01:19:13Z","timestamp":1687137553000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["One\u2010Pot Chitin Conversion to High\u2010Activity Antifungal N,N\u2010Dimethyl Chitosan Oligosaccharides"],"prefix":"10.1002","volume":"16","author":[{"given":"Jiong","family":"Cheng","sequence":"first","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering National University of Singapore  4 Engineering Drive 4 117585 Singapore"},{"name":"School of Environmental Science and Engineering State Key Lab of Metal Matrix Composites Shanghai Jiao Tong University  800 Dongchuan Road Shanghai 200240 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arunmozhiarasi","family":"Armugam","sequence":"additional","affiliation":[{"name":"Institute of Bioengineering and Bioimaging, A*STAR  31 Biopolis Way, The Nanos #07-01 Singapore 138669 Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Yang","sequence":"additional","affiliation":[{"name":"School of Environmental Science and Engineering State Key Lab of Metal Matrix Composites Shanghai Jiao Tong University  800 Dongchuan Road Shanghai 200240 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangming","family":"Jin","sequence":"additional","affiliation":[{"name":"School of Environmental Science and Engineering State Key Lab of Metal Matrix Composites Shanghai Jiao Tong University  800 Dongchuan Road Shanghai 200240 China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yugen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Bioengineering and Bioimaging, A*STAR  31 Biopolis Way, The Nanos #07-01 Singapore 138669 Singapore"},{"name":"Institute of Sustainability for Chemicals, Energy and Environment (ISCE2), A*STAR  1 Pesek Road Jurong Island, Singapore 627833 Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1877-9206","authenticated-orcid":false,"given":"Ning","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Chemical and Biomolecular Engineering National University of Singapore  4 Engineering Drive 4 117585 Singapore"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,7,20]]},"reference":[{"key":"e_1_2_8_1_1","unstructured":"\u00a0"},{"key":"e_1_2_8_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41929-018-0212-4"},{"key":"e_1_2_8_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chempr.2019.05.022"},{"key":"e_1_2_8_4_2","doi-asserted-by":"publisher","DOI":"10.1039\/C8CS00452H"},{"key":"e_1_2_8_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41570-022-00411-8"},{"key":"e_1_2_8_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.accounts.9b00573"},{"key":"e_1_2_8_7_2","doi-asserted-by":"publisher","DOI":"10.1039\/C9GC01210A"},{"key":"e_1_2_8_8_2","doi-asserted-by":"publisher","DOI":"10.1039\/D0CS00314J"},{"key":"e_1_2_8_9_1","unstructured":"\u00a0"},{"key":"e_1_2_8_10_2","doi-asserted-by":"publisher","DOI":"10.1039\/c3gc36994c"},{"key":"e_1_2_8_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/524155a"},{"key":"e_1_2_8_12_1","unstructured":"\u00a0"},{"key":"e_1_2_8_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/chem.201602389"},{"key":"e_1_2_8_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gee.2018.07.007"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/md13031133"},{"key":"e_1_2_8_16_1","unstructured":"\u00a0"},{"key":"e_1_2_8_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/acs.accounts.0c00842"},{"key":"e_1_2_8_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/cssc.202001955"},{"key":"e_1_2_8_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2023.162214"},{"key":"e_1_2_8_20_2","first-page":"1","author":"Xu B.","year":"2023","journal-title":"Front. 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