{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T17:41:26Z","timestamp":1791222086758,"version":"4.1.0"},"reference-count":83,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2024,1,22]],"date-time":"2024-01-22T00:00:00Z","timestamp":1705881600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004729","name":"Technische Universit\u00e4t Wien","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004729","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemSusChem"],"published-print":{"date-parts":[[2024,4,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Herein an enzyme\u2010assisted supercritical fluid extraction (EA\u2010SFE) was developed using the enzyme mix snailase to obtain flavonols and dihydrochalcones, subgroups of flavonoids, from globally abundant waste product apple pomace. Snailase, a commercially available mix of 20\u201330\u2005enzymes, was successfully used to remove the sugar moieties from quercetin glycosides, kaempferol glycosides, phloridzin and 3\u2010hydroxyphloridzin. The resulting flavonoid aglycones quercetin, kaempferol, phloretin and 3\u2010hydroxyphloretin were extracted using supercritical carbon dioxide (scCO\n                    <jats:sub>2<\/jats:sub>\n                    ) and minimum amounts of polar cosolvents. A sequential process of enzymatic hydrolysis and supercritical fluid extraction was developed, and the influence of the amount of snailase, pre\u2010treatment of apple pomace, the time for enzymatic hydrolysis, the amount and type of cosolvent and the time for extraction, was studied. This revealed that even small amounts of snailase (0.25\u2009%) provide a successful cleavage of sugar moieties up to 96\u2009% after 2\u2005h of enzymatic hydrolysis followed by supercritical fluid extraction with small amounts of methanol as cosolvent, leading up to 90\u2009% of the total extraction yields after 1\u2005h extraction time. Ultimately, a simultaneous process of EA\u2010SFE successfully demonstrates the potential of snailase in scalable scCO\n                    <jats:sub>2<\/jats:sub>\n                    extraction processes for dry and wet apple pomace with satisfactory enzyme activity, even under pressurized conditions.\n                  <\/jats:p>","DOI":"10.1002\/cssc.202301094","type":"journal-article","created":{"date-parts":[[2023,12,12]],"date-time":"2023-12-12T18:01:05Z","timestamp":1702404065000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Enzyme\u2010Assisted Supercritical Fluid Extraction of Flavonoids from Apple Pomace (Malus\u00d7domestica)"],"prefix":"10.1002","volume":"17","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8045-5667","authenticated-orcid":false,"given":"Philipp","family":"Mik\u0161ovsky","sequence":"first","affiliation":[{"name":"TU Wien Institute of Applied Synthetic Chemistry (E163)  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