{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T22:22:44Z","timestamp":1778624564412,"version":"3.51.4"},"reference-count":49,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2024,11,14]],"date-time":"2024-11-14T00:00:00Z","timestamp":1731542400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2024,11,14]],"date-time":"2024-11-14T00:00:00Z","timestamp":1731542400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/V004069\/1"],"award-info":[{"award-number":["BB\/V004069\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010663","name":"H2020 European Research Council","doi-asserted-by":"publisher","award":["ERC, 951231"],"award-info":[{"award-number":["ERC, 951231"]}],"id":[{"id":"10.13039\/100010663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemSusChem"],"published-print":{"date-parts":[[2025,2,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Synthetic polymers are resistant to biological attack, resulting in their long\u2010term accumulation in landfills and in natural aquatic and terrestrial habitats. Lytic polysaccharide monooxygenases (LPMOs) are enzymes which oxidatively cleave the polysaccharide chains in recalcitrant polysaccharides such as cellulose. It has been widely hypothesised that LPMOs could be used to aid in the enzymatic breakdown of synthetic polymers. Herein, through the use of biochemical assays, X\u2010ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM) we show that LPMOs can bind to polyethylene terephthalate (PET), and \u2013 in doing so \u2013 the hydrophobic surface of PET becomes more hydrophilic such that product release is boosted by subsequent treatment with classical PETases. The boosting effect is however, only observed in reactions when the LPMO and the PETase are added sequentially rather than simultaneously to the PET. Moreover, the same boosting effect is also seen when a catalytically\u2010inactive mutant of LPMO is used, showing that the principal means by which AA9 LPMOs boost the degradation of synthetic polymers is through their role as a \u201chydrophobin\u201d rather than as an oxygenase. Indeed, in accord with this role of LPMOs, we further show that this effect can be extended to other ostensibly \u2018non\u2010catalytic\u2019 proteins beyond LPMOs, such as bovine serum albumin and lactate dehydrogenase.<\/jats:p>","DOI":"10.1002\/cssc.202401350","type":"journal-article","created":{"date-parts":[[2024,11,14]],"date-time":"2024-11-14T04:37:56Z","timestamp":1731559076000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["On the Non\u2010Catalytic Role of Lytic Polysaccharide Monooxygenases in Boosting the Action of PETases on PET Polymers"],"prefix":"10.1002","volume":"18","author":[{"given":"Thamy L. R.","family":"Corr\u00eaa","sequence":"first","affiliation":[{"name":"Department of Chemistry University of York  Heslington York YO10 5DD United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ellen K. B.","family":"Rom\u00e1n","sequence":"additional","affiliation":[{"name":"Molecular and Morphofunctional Biology Graduate Program Institute of Biology University of Campinas (UNICAMP)  Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos A. R.","family":"Costa","sequence":"additional","affiliation":[{"name":"Brazilian Nanotechnology National Laboratory (LNNano) Brazilian Center for Research in Energy and Materials (CNPEM)  Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucia D.","family":"Wolf","sequence":"additional","affiliation":[{"name":"Brazilian Biorenewables National Laboratory (LNBR) Brazilian Center for Research in Energy and Materials (CNPEM)  Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Landers","sequence":"additional","affiliation":[{"name":"Institute of Physics Gleb Wataghin University of Campinas (UNICAMP)  Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Biely","sequence":"additional","affiliation":[{"name":"Institute of Chemistry Slovak Academy of Sciences  Bratislava Slovak Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario T.","family":"Murakami","sequence":"additional","affiliation":[{"name":"Brazilian Biorenewables National Laboratory (LNBR) Brazilian Center for Research in Energy and Materials (CNPEM)  Campinas Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1152-1480","authenticated-orcid":false,"given":"Paul H.","family":"Walton","sequence":"additional","affiliation":[{"name":"Department of Chemistry University of York  Heslington York YO10 5DD United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,11,14]]},"reference":[{"key":"e_1_2_9_1_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciadv.1700782"},{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aat4997"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abb0354"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-environ-112522-072642"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-08316-9"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.est.8b05083"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.envint.2020.106274"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.marpolbul.2022.113761"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aad6359"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-02277-7"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1718804115"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2006753117"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2149-4"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2021.107770"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41570-020-0163-6"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-micro-092611-150044"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1192231"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi100009p"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1105776108"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/biot.201600008"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2021.09.005"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2021.02.026"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13068-019-1449-0"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)97200-5"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/acscatal.9b00568"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-09326-3"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.20084"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.enzmictec.2020.109715"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1039\/D1SC04286F"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2017.10.014"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes10050373"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.3390\/fermentation8020047"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2017.01.089"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/ed070p25"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2005.06.015"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.702365"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.2005.04803.x"},{"key":"e_1_2_9_38_1","first-page":"447","volume":"14","author":"Scholtmeijer K.","year":"2004","journal-title":"Biomed. Mater. Eng."},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsre.2005.01.004"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1110\/ps.062326706"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.04111-14"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-29749-0"},{"key":"e_1_2_9_43_1","first-page":"1","volume-title":"Ullmann\u2018s Encyclopedia of Industrial Chemistry","author":"Gahleitner M.","year":"2003"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-022-34908-z"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12010-013-0208-0"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13068-018-1105-0"},{"key":"e_1_2_9_47_1","unstructured":"A.\u2005M. Castro A.\u2005C. de\u2005Oliveira J.\u2005N. Junior R.\u2005O.\u2005M.\u2005A. de\u2005Souza T European Patent Application2021 4249547\u2005A1."},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.enzmictec.2007.10.007"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmr.2734"}],"container-title":["ChemSusChem"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cssc.202401350","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cssc.202401350","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cssc.202401350","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T08:00:22Z","timestamp":1775808022000},"score":1,"resource":{"primary":{"URL":"https:\/\/chemistry-europe.onlinelibrary.wiley.com\/doi\/10.1002\/cssc.202401350"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11,14]]},"references-count":49,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,2,16]]}},"alternative-id":["10.1002\/cssc.202401350"],"URL":"https:\/\/doi.org\/10.1002\/cssc.202401350","archive":["Portico"],"relation":{},"ISSN":["1864-5631","1864-564X"],"issn-type":[{"value":"1864-5631","type":"print"},{"value":"1864-564X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11,14]]},"assertion":[{"value":"2024-06-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-14","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e202401350"}}