{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T15:08:16Z","timestamp":1772118496897,"version":"3.50.1"},"reference-count":50,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,4,24]],"date-time":"2025-04-24T00:00:00Z","timestamp":1745452800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2022YFA0912300"],"award-info":[{"award-number":["2022YFA0912300"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemSusChem"],"published-print":{"date-parts":[[2025,5,19]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Numerous methods have been developed to address gaseous formaldehyde pollution, but most of them cannot be applied directly to eliminate the pollution of formaldehyde in aqueous solutions. Formaldehyde in aqueous solutions can be leached from formaldehyde\u2010containing solid materials (e.\u2009g., food, wood, clothes, resins) and absorbed from gaseous formaldehyde by water. Here we developed an enzymatic cleanup technique \u2014 the reconstitution of an enzyme cocktail consisting of three coenzyme\u2010free oxidoreductases (i.\u2009e., formaldehyde dismutase, methanol oxidase, and formate oxidase) and catalase for the complete oxidation of formaldehyde. This enzyme cocktail catalyzed the reaction of formaldehyde and ambient dioxygen into carbon dioxide (CO\n                    <jats:sub>2<\/jats:sub>\n                    ) and water, which was demonstrated by the stable isotope tracer technique. Significant levels of formaldehyde were detected from aqueous solutions leached from the squid, pomfret, fabric, and curtain in the market. When this enzyme cocktail was applied to treat the leachates of contaminated samples above, formaldehyde was eliminated with degradation ratios of up to 100\u2009%. This enzymatic cleanup technique, featuring excellent biosafety (for example, degradable catalysts and non\u2010immunogenicity), independence from light, high degradation ratios, and no special equipment required, could be widely used to treat contaminated food, drinking water, and formaldehyde\u2010containing leachate.\n                  <\/jats:p>","DOI":"10.1002\/cssc.202402711","type":"journal-article","created":{"date-parts":[[2025,3,20]],"date-time":"2025-03-20T06:50:15Z","timestamp":1742453415000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Enzymatic Cleanup of Formaldehyde in Aqueous Solutions"],"prefix":"10.1002","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-3250-4252","authenticated-orcid":false,"given":"Zhenyu","family":"Zhai","sequence":"first","affiliation":[{"name":"University of Chinese Academy of Sciences  19\u2005A Yuquan Road, Shijingshan District Beijing 100049 P. R. China"},{"name":"In vitro Synthetic Biology Center Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences  32 West 7th Avenue Tianjin 300308 P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunjie","family":"Li","sequence":"additional","affiliation":[{"name":"In vitro Synthetic Biology Center Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences  32 West 7th Avenue Tianjin 300308 P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhilei","family":"Li","sequence":"additional","affiliation":[{"name":"In vitro Synthetic Biology Center Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences  32 West 7th Avenue Tianjin 300308 P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingting","family":"Wang","sequence":"additional","affiliation":[{"name":"In vitro Synthetic Biology Center Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences  32 West 7th Avenue Tianjin 300308 P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuan","family":"Li","sequence":"additional","affiliation":[{"name":"In vitro Synthetic Biology Center Tianjin Institute of Industrial Biotechnology Chinese Academy of Sciences  32 West 7th Avenue Tianjin 300308 P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4010-2250","authenticated-orcid":false,"given":"Yi\u2010Heng P. Job","family":"Zhang","sequence":"additional","affiliation":[{"name":"University of Chinese Academy of Sciences  19\u2005A Yuquan Road, Shijingshan District Beijing 100049 P. R. 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