{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T20:23:06Z","timestamp":1780777386307,"version":"3.54.1"},"reference-count":22,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T00:00:00Z","timestamp":1757548800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemSusChem"],"published-print":{"date-parts":[[2025,11,4]]},"abstract":"<jats:p>\n                    Microbial electrosynthesis (MES) holds great promise for converting carbon dioxide (CO\n                    <jats:sub>2<\/jats:sub>\n                    ) into building blocks of the (bio)chemical industry. Its advancement is hindered by limited process control and an incomplete understanding of the oxygen (O\n                    <jats:sub>2<\/jats:sub>\n                    ) stress response of biocatalysts or key engineering parameters like the availability of hydrogen (H\n                    <jats:sub>2<\/jats:sub>\n                    ). With\n                    <jats:italic>Clostridium ljungdahlii<\/jats:italic>\n                    as a model acetogen for strict anaerobic MES from CO\n                    <jats:sub>2<\/jats:sub>\n                    , the effect of O\n                    <jats:sub>2<\/jats:sub>\n                    stress and H\n                    <jats:sub>2<\/jats:sub>\n                    availability using 1\u2010L electrobioreactors is showcased, providing high process control and relevance for follow\u2010up engineering and scaling. Using a combinatorial approach of two cathode materials, three anode types, and various current regimes ranging from \u22125 to \u221280\u2009mA, MES performance is boosted by overcoming O\n                    <jats:sub>2<\/jats:sub>\n                    stress and insufficient H\n                    <jats:sub>2<\/jats:sub>\n                    distribution at high current. It is demonstrated that a large\u2010surface\u2010area carbon fiber fabric cathode combined with O\n                    <jats:sub>2<\/jats:sub>\n                    evolution anodes flushed with nitrogen (N\n                    <jats:sub>2<\/jats:sub>\n                    ) allows the highest reproducible acetate concentration of 12.44\u2009\u00b1\u20091.56\u2009g\u2009L\n                    <jats:sup>\u22121<\/jats:sup>\n                    and maximum acetate production rate of 0.6\u2009\u00b1\u20090.1\u2009g\u2009L\n                    <jats:sup>\u22121<\/jats:sup>\n                    \u2009d\n                    <jats:sup>\u22121<\/jats:sup>\n                    reported for MES from CO\n                    <jats:sub>2<\/jats:sub>\n                    using a pure culture. There is certainly room for improved process control at this and even larger scales, showing that the ceiling of strict anaerobic MES is far from being reached.\n                  <\/jats:p>","DOI":"10.1002\/cssc.202501118","type":"journal-article","created":{"date-parts":[[2025,9,11]],"date-time":"2025-09-11T17:19:33Z","timestamp":1757611173000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Reducing Oxygen Stress and Improving Hydrogen Availability Boosts Microbial Electrosynthesis by\n                    <i>Clostridium<\/i>\n                    <i>ljungdahlii<\/i>"],"prefix":"10.1002","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6287-1974","authenticated-orcid":false,"given":"Anne","family":"Kuchenbuch","sequence":"first","affiliation":[{"name":"Department of Microbial Biotechnology Helmholtz\u2010Centre for Environmental Research GmbH\u2013UFZ  Permoserstr. 15 04318 Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6871-5326","authenticated-orcid":false,"given":"Sara","family":"Al\u2010Sbei","sequence":"additional","affiliation":[{"name":"Department Bio Pilot Plant Leibniz Institute for Natural Product Research and Infection Biology\u2013Hans\u2010Kn\u00f6ll\u2010Institute  Beutenbergstr. 11a 07745 Jena Germany"},{"name":"Faculty of Biological Science Friedrich Schiller University Jena  Bachstr. 18k 07743 Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5676-1958","authenticated-orcid":false,"given":"Luis F. M.","family":"Rosa","sequence":"additional","affiliation":[{"name":"Department of Microbial Biotechnology Helmholtz\u2010Centre for Environmental Research GmbH\u2013UFZ  Permoserstr. 15 04318 Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7723-6260","authenticated-orcid":false,"given":"Santiago T.","family":"Boto","sequence":"additional","affiliation":[{"name":"Department Bio Pilot Plant Leibniz Institute for Natural Product Research and Infection Biology\u2013Hans\u2010Kn\u00f6ll\u2010Institute  Beutenbergstr. 11a 07745 Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8311-338X","authenticated-orcid":false,"given":"Martin","family":"Westermann","sequence":"additional","affiliation":[{"name":"Electron Microscopy Center Jena University Hospital  Ziegelm\u00fchlenweg 1 07743 Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4566-8624","authenticated-orcid":false,"given":"Miriam A.","family":"Rosenbaum","sequence":"additional","affiliation":[{"name":"Department Bio Pilot Plant Leibniz Institute for Natural Product Research and Infection Biology\u2013Hans\u2010Kn\u00f6ll\u2010Institute  Beutenbergstr. 11a 07745 Jena Germany"},{"name":"Faculty of Biological Science Friedrich Schiller University Jena  Bachstr. 18k 07743 Jena Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0014-4640","authenticated-orcid":false,"given":"Falk","family":"Harnisch","sequence":"additional","affiliation":[{"name":"Department of Microbial Biotechnology Helmholtz\u2010Centre for Environmental Research GmbH\u2013UFZ  Permoserstr. 15 04318 Leipzig Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,9,11]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"397","volume":"367","author":"Zimmerman J. 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