{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T16:01:09Z","timestamp":1790092869955,"version":"4.0.1"},"reference-count":64,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,3,15]],"date-time":"2026-03-15T00:00:00Z","timestamp":1773532800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,15]],"date-time":"2026-03-15T00:00:00Z","timestamp":1773532800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003246","name":"Dutch Research Council","doi-asserted-by":"publisher","award":["Grant OCENW.XS24.3.183"],"award-info":[{"award-number":["Grant OCENW.XS24.3.183"]}],"id":[{"id":"10.13039\/501100003246","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemSusChem"],"published-print":{"date-parts":[[2026,3,27]]},"abstract":"<jats:p>\n                    Amide bond formation is a key transformation in organic synthesis, especially for the preparation of active pharmaceutical ingredients (APIs). In this work, we report the development of a bio\u2010organocatalytic cascade, combining stereoselective transamination catalyzed by\n                    <jats:italic>\u03c9<\/jats:italic>\n                    \u2010transaminases (\n                    <jats:italic>\u03c9<\/jats:italic>\n                    \u2010TAs) in neat organic solvent and choline chloride (ChCl)\u2010mediated direct amidation. This strategy enables the synthesis of chiral amides from prochiral carbonyl compounds and carboxylic acids under solvent\u2010free microwave conditions. After optimizing the biocatalytic transamination in MTBE, we applied the method to the synthesis of key intermediates of Racecadotril and AVR\u201048, achieving full conversions and enantiomeric excess above 99%. The amidation step, promoted by ChCl without traditional activating agents, proved highly efficient for a wide range of aliphatic and aromatic carboxylic acids, affording the target amides in 60%\u201386% yields. Solvent evaporation after the transamination step was essential to remove interfering byproducts such as acetone, thus improving amidation yields. Overall, this integrated methodology provides a green, efficient, and scalable route to access amide\u2010based building blocks in high optical purity, opening new avenues for sustainable pharmaceutical manufacturing.\n                  <\/jats:p>","DOI":"10.1002\/cssc.202501501","type":"journal-article","created":{"date-parts":[[2026,3,15]],"date-time":"2026-03-15T20:24:41Z","timestamp":1773606281000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Stereoselective Bio\u2010Organocatalytic Cascade to Chiral Amides as Active Pharmaceutical Ingredient Intermediates Using\n                    <i>\u03c9<\/i>\n                    \u2010Transaminase and Choline Chloride Under Microwave Irradiation"],"prefix":"10.1002","volume":"19","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-7351-8096","authenticated-orcid":false,"given":"Salvatore","family":"Romano","sequence":"first","affiliation":[{"name":"Van \u2019t Hoff Institute for Molecular Sciences HIMS\u2010Biocat University of Amsterdam  Amsterdam The Netherlands"},{"name":"Dipartimento di Scienze della Salute Universit\u00e0 \u201cMagna Gracia\u201d di Catanzaro  Loc. Germaneto Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-9135-5916","authenticated-orcid":false,"given":"Matteo","family":"Damian","sequence":"additional","affiliation":[{"name":"Van \u2019t Hoff Institute for Molecular Sciences HIMS\u2010Biocat University of Amsterdam  Amsterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6617-9320","authenticated-orcid":false,"given":"Monica","family":"Nardi","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze della Salute Universit\u00e0 \u201cMagna Gracia\u201d di Catanzaro  Loc. Germaneto Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0225-033X","authenticated-orcid":false,"given":"Antonio","family":"Procopio","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze della Salute Universit\u00e0 \u201cMagna Gracia\u201d di Catanzaro  Loc. 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