{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:39:15Z","timestamp":1786977555621,"version":"build-2736575974"},"reference-count":23,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T00:00:00Z","timestamp":1760486400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemSusChem"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:p>\n                    Succinic acid (SA)\/1,4\u2010Butanedioic acid is a key platform\u2010chemical with broad industrial relevance, yet its biocatalytic production is constrained by redox imbalance, by\u2010product accumulation, and limited CO\n                    <jats:sub>2<\/jats:sub>\n                    sequestration. This study overcomes the above limitations and enhanced the SA production (0.6\u2009g\u2009g\n                    <jats:sup>\u22121<\/jats:sup>\n                    ; 6\u2009g\u2009L\n                    <jats:sup>\u22121<\/jats:sup>\n                    ) by dual\u2010species catalysis using\n                    <jats:italic>Citrobacter amalonaticus<\/jats:italic>\n                    (NCIM 5782, CA) and\n                    <jats:italic>Bacillus subtilis<\/jats:italic>\n                    (BS, NCIM 5781), in a bioelectrocatalytic system. Comprehensive gene\u2010expression\u2010profiling revealed upregulation of phosphoenolpyruvate carboxylase (\n                    <jats:italic>ppc<\/jats:italic>\n                    \/PPC) in CA and pyruvate carboxylase (\n                    <jats:italic>pyc<\/jats:italic>\n                    \/PYC) in BS, reflecting intensified carboxylation activity via the reductive tricarboxylic acid pathway. Protein\/structural modeling\/docking of PPC and PYC demonstrated enhanced catalytic\u2010site exposure under electro\u2010fermentative co\u2010culture conditions, correlating with greater carboxylation efficacy. Notably, beyond extracellular\u2010CO\n                    <jats:sub>2<\/jats:sub>\n                    fixation, intracellular\u2010CO\n                    <jats:sub>2<\/jats:sub>\n                    sequestration is also evident, as indicated by a marked enrichment of H\n                    <jats:sub>2<\/jats:sub>\n                    in the biogas produced during dual\u2010species\u2010catalysis compared to monoculture systems. Thermodynamic and electrochemical evaluation indicated greater stability and electron flow in co\u2010culture reactors (R9:\u0394G\u2009=\u2009\u221242.29\u2009kJ) compared to monocultures. Collectively, this study presents a scalable hybrid bioelectrochemical strategy leveraging species\u2010specific metabolic roles and electron\u2010steering to facilitate high\u2010yield, selective SA production, offering a promising blueprint for sustainable carbon\u2010based biomanufacturing.\n                  <\/jats:p>","DOI":"10.1002\/cssc.202501802","type":"journal-article","created":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T12:59:19Z","timestamp":1760533159000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Electro\u2010Stimulated Dual\u2010Species Catalysis Enables CO\n                    <sub>2<\/sub>\n                    Fixation Toward Selective 1,4\u2010Butanedioic Acid Biosynthesis"],"prefix":"10.1002","volume":"18","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5710-5493","authenticated-orcid":false,"given":"Triya","family":"Mukherjee","sequence":"first","affiliation":[{"name":"Bioengineering and Environmental Sciences Lab, Department of Energy and Environmental Engineering CSIR\u2010Indian Institute of Chemical Technology (CSIR\u2010IICT)  Hyderabad 500007 India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Ghaziabad 201002 India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5564-4135","authenticated-orcid":false,"given":"Venkata Mohan","family":"S.","sequence":"additional","affiliation":[{"name":"Bioengineering and Environmental Sciences Lab, Department of Energy and Environmental Engineering CSIR\u2010Indian Institute of Chemical Technology (CSIR\u2010IICT)  Hyderabad 500007 India"},{"name":"Academy of Scientific and Innovative Research (AcSIR)  Ghaziabad 201002 India"},{"name":"Director's Office CSIR\u2010National Environmental Engineering Research Institute  Nagpur 440020 India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,15]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2019.133838"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fbioe.2022.843887"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2005.03.001"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2004.03.001"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20080709"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.chemrev.2c00581"},{"key":"e_1_2_7_8_1","first-page":"140005","author":"Yang Y.","year":"2023","journal-title":"J. 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