{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T05:12:16Z","timestamp":1791781936514,"version":"4.3.4"},"reference-count":20,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T00:00:00Z","timestamp":1773878400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T00:00:00Z","timestamp":1773878400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100008398","name":"Villum Foundation","doi-asserted-by":"publisher","award":["Grant 40815"],"award-info":[{"award-number":["Grant 40815"]}],"id":[{"id":"10.13039\/100008398","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemSusChem"],"published-print":{"date-parts":[[2026,3,27]]},"abstract":"<jats:p>\n                    Recycling of enzymatically depolymerized poly(ethylene terephthalate) (PET) involves polycondensation of bis(2\u2010hydroxy\u2010ethyl) terephthalic acid (BHET)\u2014a degradation product of enzymatic PET hydrolysis. The recycling process is simplified when more BHET is generated by the enzymatic reaction. Here, we report how ethylene glycol (EG) addition can maximize BHET formation using leading PET hydrolases, LCC\n                    <jats:sub>ICCG<\/jats:sub>\n                    , and PHL7. EG at any level above 2\u20135% vol\/vol was found to decrease the steady\u2010state enzymatic degradation rates while enhancing the relative production of BHET. For LCC\n                    <jats:sub>ICCG<\/jats:sub>\n                    , the highest measured BHET levels (product fraction approaching 0.5) were attained at EG levels of \u223c27\u201329% and reaction temperature \u223c62.5\u00b0C. EG shortened the enzymatic reaction lag\u2010phase and lowered the lag\u2010phase increase with PET crystallinity. EG works by perturbing the adsorption, including nonproductive adsorption, of the enzymes to the PET surface, which manifests as an apparent change in substrate affinity (increases the\n                    <jats:sup>inv<\/jats:sup>\n                    <jats:italic>K<\/jats:italic>\n                    <jats:sub>m<\/jats:sub>\n                    in interfacial kinetics modeling) and directs the enzyme more to the liquid phase.\n                  <\/jats:p>","DOI":"10.1002\/cssc.202502346","type":"journal-article","created":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T08:21:44Z","timestamp":1773908504000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Ethylene Glycol\u2010Guided Enhancement of Bis(2\u2010Hydroxyethyl) Terephthalic Acid as a Primary Product of Enzymatic Poly(Ethylene Terephthalate) Depolymerization"],"prefix":"10.1002","volume":"19","author":[{"given":"Tobias S.","family":"Radmer","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Biomedicine DTU Bioengineering Technical University of Denmark  Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thore B.","family":"Thomsen","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Biomedicine DTU Bioengineering Technical University of Denmark  Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leon T.","family":"Krinn","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Biomedicine DTU Bioengineering Technical University of Denmark  Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8910-9931","authenticated-orcid":false,"given":"Anne S.","family":"Meyer","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Biomedicine DTU Bioengineering Technical University of Denmark  Lyngby Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,19]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cscee.2024.100673"},{"key":"e_1_2_11_3_1","unstructured":"Plastics Europe \u201cPlastics \u2010 the Facts 2024 \u201d accessed 2024 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