{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,12]],"date-time":"2026-09-12T04:10:06Z","timestamp":1789186206117,"version":"build-2803163510"},"reference-count":27,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T00:00:00Z","timestamp":1788048000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,8,30]],"date-time":"2026-08-30T00:00:00Z","timestamp":1788048000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2023YFC3403600"],"award-info":[{"award-number":["2023YFC3403600"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["chemistry-europe.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["ChemSusChem"],"published-print":{"date-parts":[[2026,9,14]]},"abstract":"<jats:p>\n                    Engineered microbial systems offer a route for converting lignin into value\u2010added aromatics, yet lignin\u2010derived intermediates are cytotoxic and suppress growth and metabolic flux. Here, we show that ectoine, a compatible solute, mitigates lignin\u2010induced stress in\n                    <jats:italic>Halalkalibacterium ligniniphilum<\/jats:italic>\n                    L1 and improves bioconversion performance. At 0.1\u2009g L\n                    <jats:sup>\u22121<\/jats:sup>\n                    , ectoine alleviated surface damage, improved chemical oxygen demand (COD) removal from about 40% to about 55%, and increased decolorization from about 25% to about 48%\u201350% over 7 days. Assays using crude extracts from recombinant\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    expressing individual L1\u2010derived enzymes showed that ectoine was associated with broader apparent pH and temperature operating ranges, up to a 12.3% point increase in fixed time substrate conversion, and improved time\u2010dependent retention of measurable activity. Transcriptomic analysis identified 24 differentially expressed genes (9 upregulated and 15 downregulated; false discovery rate (FDR)\u2009&lt;\u20090.05, |log\n                    <jats:sub>2<\/jats:sub>\n                    fold change (log\n                    <jats:sub>2<\/jats:sub>\n                    \u2009FC) | \u2265\u20091), indicating a focused rather than global response. Ectoine\u2010responsive changes were associated with membrane\/envelope\u2010related functions, transport, and oxidoreductase\u2010related processes, whereas motility and generalized stress\u2010associated programs were reduced. In a 5\u2010L bioreactor, ectoine increased vanillin titers by 13.9%\u201323.0% at lignin concentrations of 20\u201340\u2009g L\n                    <jats:sup>\u22121<\/jats:sup>\n                    . These results support ectoine as an additive for lignin bioconversion under stress.\n                  <\/jats:p>","DOI":"10.1002\/cssc.71032","type":"journal-article","created":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T03:24:46Z","timestamp":1788146686000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Ectoine Mitigates Stress and Enhances Lignin to Vanillin Bioconversion in\n                    <i>Halalkalibacterium ligniniphilum<\/i>\n                    L1"],"prefix":"10.1002","volume":"19","author":[{"given":"Yue","family":"Bai","sequence":"first","affiliation":[{"name":"International Joint Laboratory on Synthetic Biology and Biomass Biorefinery Biofuels Institute School of the Environment and Safety Engineering Jiangsu University  Zhenjiang China"},{"name":"Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment Suzhou University of Science and Technology  Suzhou 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University of Science and Technology  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9646-9556","authenticated-orcid":false,"given":"Daochen","family":"Zhu","sequence":"additional","affiliation":[{"name":"International Joint Laboratory on Synthetic Biology and Biomass Biorefinery Biofuels Institute School of the Environment and Safety Engineering Jiangsu University  Zhenjiang China"},{"name":"Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment Suzhou University of Science and Technology  Suzhou 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