{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T16:51:40Z","timestamp":1783529500940,"version":"3.55.0"},"reference-count":74,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,2,29]],"date-time":"2020-02-29T00:00:00Z","timestamp":1582934400000},"content-version":"vor","delay-in-days":59,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["13GW0193B"],"award-info":[{"award-number":["13GW0193B"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin &amp; Trans Imm"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Primary open\u2010angle glaucoma (POAG) is a neurodegenerative disorder leading to a gradual vision loss caused by progressive damage to the optic nerve. Immunological processes are proposed to be involved in POAG pathogenesis. Altered serological autoantibody levels have been frequently reported, but complete analyses of the natural autoantibodies with respect to disease\u2010related alterations are scarce. Here, we provide an explorative analysis of pathways and biological processes that may involve naturally immunogenic proteins and highlight POAG\u2010specific alterations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Mass spectrometry\u2010based antibody\u2010mediated identification of autoantigens (MS\u2010AMIDA) was carried out in healthy and glaucomatous trabecular meshwork (TM) cell lines, using antibody pools purified from serum samples of 30 POAG patients and 30 non\u2010glaucomatous subjects. Selected antigens were validated by protein microarray (<jats:italic>n<\/jats:italic>\u00a0=\u00a0120). Bioinformatic assessment of identified autoantigens, including Gene Ontology (GO) enrichment analysis and protein\u2013protein interaction networks, was applied.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Overall, we identified 106 potential autoantigens [false discovery rate (FDR)\u00a0&lt;\u00a00.01], from which we considered 66 as physiological targets of natural autoantibodies. Twenty\u2010one autoantigens appeared to be related to POAG. Bioinformatic analysis revealed that the platelet\u2010derived growth factor receptor beta (PDGFRB) pathway involved in TM fibrosis was particularly rich in POAG\u2010related antigens. Antibodies to threonine\u2010tRNA ligase (TARS), component 1 Q subcomponent\u2010binding protein (C1QBP) and paraneoplastic antigen Ma2 (PNMA2) showed significantly (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) higher levels in POAG patients as validated by protein microarray.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study provides new insights into autoimmunity in health and glaucoma. Bioinformatic analysis of POAG\u2010related autoantigens showed a strong association with the PDGFRB pathway and also increased levels of PNMA2, TARS, and C1QBP autoantibodies in the serum of POAG patients as potential glaucoma biomarkers.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cti2.1101","type":"journal-article","created":{"date-parts":[[2020,2,29]],"date-time":"2020-02-29T12:09:51Z","timestamp":1582978191000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Autoantigens in the trabecular meshwork and glaucoma\u2010specific alterations in the natural autoantibody repertoire"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3354-2020","authenticated-orcid":false,"given":"Vanessa M","family":"Beutgen","sequence":"first","affiliation":[{"name":"Experimental and Translational Ophthalmology Department of Ophthalmology University Medical Center of the Johannes Gutenberg \u2010 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