{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T15:14:05Z","timestamp":1787670845374,"version":"build-2736575974"},"reference-count":53,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,4,29]],"date-time":"2021-04-29T00:00:00Z","timestamp":1619654400000},"content-version":"vor","delay-in-days":118,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002704","name":"Fundaci\u00f3n Cient\u00edfica Asociaci\u00f3n Espa\u00f1ola Contra el C\u00e1ncer","doi-asserted-by":"publisher","award":["AECC\u2010AIO2017"],"award-info":[{"award-number":["AECC\u2010AIO2017"]}],"id":[{"id":"10.13039\/501100002704","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin &amp;amp; Trans Imm"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Adoptive cell therapy (ACT) with mature T cells modified with a chimeric antigen receptor\u00a0has demonstrated improved outcome for B\u2010cell malignancies. However, its application for others such as Hodgkin lymphoma remains a clinical challenge. CD30 antigen, expressed in Hodgkin lymphoma cells, is absent in most healthy tissues, representing an ideal target of ACT for this disease. Despite that, efficacy of CD30\u2010chimeric antigen receptor (CAR) T cells for Hodgkin lymphoma remains modest. Here, we have developed and tested a novel CD30\u2010CAR T to improve efficacy of CD30\u2010CAR therapy, using a targeting epitope within the non\u2010cleavable part of CD30 receptor, and memory stem T cells (T<jats:sub>SCM<\/jats:sub>) to improve engraftment, persistence and antitumor activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>T<jats:sub>SCM\u2010like<\/jats:sub> cultures were generated and expanded <jats:italic>ex vivo<\/jats:italic> and transduced at day 1 or 2 with a lentiviral vector encoding the CD30\u2010CAR. Therapeutic <jats:italic>in vivo<\/jats:italic> experiments were performed using NSG mice injected with L540 (sc) or L428 (iv) and treated with CD30\u2010CAR T cells when the tumor was established.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CD30\u2010CAR T<jats:sub>SCM\u2010like<\/jats:sub> cells generated and expanded <jats:italic>ex vivo<\/jats:italic>, despite CD30 expression and fratricide killing of CD30<jats:sup>+<\/jats:sup> CAR T cells, were not impaired by soluble CD30 and completely eradicated Hodgkin lymphoma <jats:italic>in vivo<\/jats:italic>, showing high persistence and long\u2010lasting immunity. In addition, highly enriched CD30\u2010CAR T<jats:sub>SCM\u2010like<\/jats:sub> products confer a survival advantage <jats:italic>in vivo<\/jats:italic>, in contrast to more differentiated CAR T cells, with higher tumor infiltration and enhanced antitumor effect.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study supports the use of a refined CD30\u2010CAR T cells with highly enriched T<jats:sub>SCM\u2010like<\/jats:sub> products to improve clinical efficacy of CAR T for Hodgkin lymphoma.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cti2.1268","type":"journal-article","created":{"date-parts":[[2021,4,29]],"date-time":"2021-04-29T12:22:32Z","timestamp":1619698952000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Memory stem T cells modified with a redesigned CD30\u2010chimeric antigen receptor show an enhanced antitumor effect in Hodgkin lymphoma"],"prefix":"10.1002","volume":"10","author":[{"given":"Carmen","family":"Alvarez\u2010Fern\u00e1ndez","sequence":"first","affiliation":[{"name":"Hematology Service Hospital de la Santa Creu y Sant Pau  Barcelona Spain"},{"name":"Laboratory of Experimental Hematology\u2010IIB Institut Recerca Hospital de la Santa Creu y Sant Pau  Barcelona Spain"},{"name":"Jos\u00e9 Carreras Leukemia Research Institute  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Escrib\u00e0\u2010Garcia","sequence":"additional","affiliation":[{"name":"Hematology Service Hospital de la Santa Creu y Sant Pau  Barcelona Spain"},{"name":"Laboratory of Experimental Hematology\u2010IIB Institut Recerca Hospital de la Santa Creu y Sant Pau  Barcelona Spain"},{"name":"Jos\u00e9 Carreras Leukemia Research Institute  Barcelona 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