{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T18:41:31Z","timestamp":1790707291556,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2021,10,27]],"date-time":"2021-10-27T00:00:00Z","timestamp":1635292800000},"content-version":"vor","delay-in-days":299,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin &amp; Trans Imm"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>A diverse intratumoral T\u2010cell receptor (TCR) repertoire is associated with improved survival in diffuse large B\u2010cell lymphoma (DLBCL) treated\u2009with rituximab\/cyclophosphamide\/doxorubicin\/prednisolone\/vincristine (R\u2010CHOP) chemoimmunotherapy. We explored the impact of intratumoral TCR repertoire on interim PET (iPET) done after four cycles of R\u2010CHOP, the relationships between intratumoral and circulating repertoire, and the phenotypes of expanded clonotypes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We sequenced the third complementarity\u2010determining region of TCR\u03b2 in tumor samples, blood at pre\u2010therapy and after four cycles of R\u2010CHOP in 35 patients enrolled in ALLGNHL21 trial in high\u2010risk DLBCL. We correlated the TCR diversity metrics with iPET status, gene expression profiles and HLA\u2010class I genotypes. We then sequenced the FACS\u2010sorted peripheral blood T cells in six patients, and pentamer\u2010sorted EBV\u2010specific CD8<jats:sup>+<\/jats:sup> T cells in one patient from this cohort.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared with iPET<jats:sup>\u2212<\/jats:sup> patients, the intratumoral TCR repertoire in iPET<jats:sup>+<\/jats:sup> patients was characterised by higher cumulative frequency of abundant clonotypes and higher productive clonality. There was a variable overlap between circulating and intratumoral repertoires, with the dominant intratumoral clonotypes more likely to be detected in the blood. The majority of shared clonotypes were CD8<jats:sup>+<\/jats:sup> PD\u20101<jats:sup>HI<\/jats:sup> T cells, and CD8<jats:sup>+<\/jats:sup> T cells had the largest clonal expansions in tumor and blood. In a patient with EBV<jats:sup>+<\/jats:sup> DLBCL, EBV\u2010specific intratumoral clonotypes were trackable in the blood.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study demonstrates that clonally expanded intratumoral TCR repertoires are associated with iPET<jats:sup>+<\/jats:sup> and that the blood can be used to track tumor\u2010associated antigen\u2010specific clonotypes. These findings assist the rationale design and therapeutic monitoring of immunotherapeutic strategies in DLBCL.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cti2.1351","type":"journal-article","created":{"date-parts":[[2021,10,27]],"date-time":"2021-10-27T07:56:30Z","timestamp":1635321390000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Intratumoral T\u2010cell receptor repertoire is predictive of interim PET scan results in patients with diffuse large B\u2010cell lymphoma treated with rituximab\/cyclophosphamide\/doxorubicin\/prednisolone\/vincristine (R\u2010CHOP) chemoimmunotherapy"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6558-1721","authenticated-orcid":false,"given":"Mohamed","family":"Shanavas","sequence":"first","affiliation":[{"name":"Mater Research University of Queensland Brisbane QLD Australia"},{"name":"Department of Haematology Mater Hospital Brisbane QLD 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