{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T08:39:12Z","timestamp":1791362352223,"version":"4.3.1"},"reference-count":63,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,2,2]],"date-time":"2022-02-02T00:00:00Z","timestamp":1643760000000},"content-version":"vor","delay-in-days":32,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin &amp; Trans Imm"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Autologous chimeric antigen receptor (CAR) \u03b1\u03b2 T\u2010cell therapies have demonstrated remarkable antitumor efficacy in patients with haematological malignancies; however, not all eligible cancer patients receive clinical benefit. Emerging strategies to improve patient access and clinical responses include using premanufactured products from healthy donors and alternative cytotoxic effectors possessing intrinsic tumoricidal activity as sources of CAR cell therapies. \u03b3\u03b4 T cells, which combine innate and adaptive mechanisms to recognise and kill malignant cells, are an attractive candidate platform for allogeneic CAR T\u2010cell therapy. Here, we evaluated the manufacturability and functionality of allogeneic peripheral blood\u2010derived CAR<jats:sup>+<\/jats:sup> V\u03b41 \u03b3\u03b4 T cells expressing a second\u2010generation CAR targeting the B\u2010cell\u2010restricted CD20 antigen.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Donor\u2010derived V\u03b41 \u03b3\u03b4 T cells from peripheral blood were <jats:italic>ex\u2009vivo<\/jats:italic>\u2010activated, expanded and engineered to express a novel anti\u2010CD20 CAR. <jats:italic>In vitro<\/jats:italic> and <jats:italic>in\u2009vivo<\/jats:italic> assays were used to evaluate CAR\u2010dependent and CAR\u2010independent antitumor activities of CD20 CAR<jats:sup>+<\/jats:sup> V\u03b41 \u03b3\u03b4 T cells against B\u2010cell tumors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Anti\u2010CD20 CAR<jats:sup>+<\/jats:sup> V\u03b41 \u03b3\u03b4 T cells exhibited innate and adaptive antitumor activities, such as <jats:italic>in\u2009vitro<\/jats:italic> tumor cell killing and proinflammatory cytokine production, in addition to <jats:italic>in\u2009vivo<\/jats:italic> tumor growth inhibition of B\u2010cell lymphoma xenografts in immunodeficient mice. Furthermore, CD20 CAR<jats:sup>+<\/jats:sup> V\u03b41 \u03b3\u03b4 T cells did not induce xenogeneic graft\u2010versus\u2010host disease in immunodeficient mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These preclinical data support the clinical evaluation of ADI\u2010001, an allogeneic CD20 CAR<jats:sup>+<\/jats:sup> V\u03b41 \u03b3\u03b4 T cell, and a phase 1 study has been initiated in patients with B\u2010cell malignancies (NCT04735471).<\/jats:p><\/jats:sec>","DOI":"10.1002\/cti2.1373","type":"journal-article","created":{"date-parts":[[2022,2,2]],"date-time":"2022-02-02T17:00:57Z","timestamp":1643821257000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":118,"title":["Allogeneic CD20\u2010targeted \u03b3\u03b4 T cells exhibit innate and adaptive antitumor activities in preclinical B\u2010cell lymphoma models"],"prefix":"10.1002","volume":"11","author":[{"given":"Kevin P","family":"Nishimoto","sequence":"first","affiliation":[{"name":"Adicet Bio, Inc. Menlo Park CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taylor","family":"Barca","sequence":"additional","affiliation":[{"name":"Adicet Bio, Inc. Menlo Park CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aruna","family":"Azameera","sequence":"additional","affiliation":[{"name":"Adicet Bio, Inc. Menlo Park CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amani","family":"Makkouk","sequence":"additional","affiliation":[{"name":"Adicet Bio, Inc. Menlo Park CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason M","family":"Romero","sequence":"additional","affiliation":[{"name":"Adicet Bio, Inc. Menlo Park CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Bai","sequence":"additional","affiliation":[{"name":"Adicet Bio, Inc. Menlo Park CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary M","family":"Brodey","sequence":"additional","affiliation":[{"name":"Adicet Bio, Inc. Menlo Park CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jackie","family":"Kennedy\u2010Wilde","sequence":"additional","affiliation":[{"name":"Adicet Bio, Inc. Menlo Park CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Shao","sequence":"additional","affiliation":[{"name":"Adicet Bio, Inc. Menlo Park CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie","family":"Papaioannou","sequence":"additional","affiliation":[{"name":"Adicet Bio, Inc. Menlo Park CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy","family":"Doan","sequence":"additional","affiliation":[{"name":"Adicet Bio, Inc. Menlo Park CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cynthia","family":"Masri","sequence":"additional","affiliation":[{"name":"Adicet Bio, Inc. Menlo Park CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ngoc T","family":"Hoang","sequence":"additional","affiliation":[{"name":"Adicet Bio, Inc. Menlo Park CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hayden","family":"Tessman","sequence":"additional","affiliation":[{"name":"Adicet Bio, Inc. Menlo Park CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vidhya Dhevi","family":"Ramanathan","sequence":"additional","affiliation":[{"name":"Adicet Bio, Inc. Menlo Park CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana","family":"Giner\u2010Rubio","sequence":"additional","affiliation":[{"name":"Adicet Bio, Inc. Menlo Park CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Delfino","sequence":"additional","affiliation":[{"name":"Regeneron Pharmaceuticals, Inc. Tarrytown NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kriti","family":"Sharma","sequence":"additional","affiliation":[{"name":"Regeneron Pharmaceuticals, Inc. Tarrytown NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Bray","sequence":"additional","affiliation":[{"name":"Regeneron Pharmaceuticals, Inc. Tarrytown NY USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Hoopes","sequence":"additional","affiliation":[{"name":"Adicet Bio, Inc. Menlo Park CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daulet","family":"Satpayev","sequence":"additional","affiliation":[{"name":"Adicet Bio, Inc. Menlo Park CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ranjita","family":"Sengupta","sequence":"additional","affiliation":[{"name":"Adicet Bio, Inc. Menlo Park CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marissa","family":"Herrman","sequence":"additional","affiliation":[{"name":"Adicet Bio, Inc. Menlo Park CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stewart E","family":"Abbot","sequence":"additional","affiliation":[{"name":"Adicet Bio, Inc. Menlo Park CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4020-2718","authenticated-orcid":false,"given":"Blake T","family":"Aftab","sequence":"additional","affiliation":[{"name":"Adicet Bio, Inc. Menlo Park CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zili","family":"An","sequence":"additional","affiliation":[{"name":"Adicet Bio, Inc. Menlo Park CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Swapna","family":"Panuganti","sequence":"additional","affiliation":[{"name":"Adicet Bio, Inc. Menlo Park CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra M","family":"Hayes","sequence":"additional","affiliation":[{"name":"Adicet Bio, Inc. Menlo Park CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,2,2]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.beha.2018.03.001"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41571-019-0184-6"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40259-017-0247-0"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells7100155"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/acg2.86"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2018.36.15_suppl.e15042"},{"issue":"13","key":"e_1_2_9_8_1","article-title":"Functional demonstration of CD19 chimeric antigen receptor (CAR) engineered Epstein\u2010Barr virus (EBV) specific T cells: an off\u2010the\u2010shelf, allogeneic CAR T\u2010cell immunotherapy platform","volume":"79","author":"Shen RR","year":"2019","journal-title":"Cancer Res"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-10-3236"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1702830"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(85)90140-0"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/322145a0"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2781"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3909"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.aax9364"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2014.00607"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2014.104"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2014.00636"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbmt.2014.09.027"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2019.01342"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2017-07-793760"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.01367"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1324022111"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep17943"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms14760"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.1158\/2326-6066.CIR-18-0647"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1158\/2326-6066.CIR-14-0186"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3838"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2017.09.015"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2012.00115"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-018-0010-1"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2015.247"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2017.35.15_suppl.3023"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1818488116"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41573-019-0038-z"},{"key":"e_1_2_9_36_1","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevImmunol.v23.i56.10"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3774"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.2015.139493"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2017.01736"},{"key":"e_1_2_9_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3862"},{"key":"e_1_2_9_41_1","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.aax1872"},{"key":"e_1_2_9_42_1","doi-asserted-by":"publisher","DOI":"10.1186\/1746-1596-6-33"},{"key":"e_1_2_9_43_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12185-009-0295-4"},{"key":"e_1_2_9_44_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1008416911099"},{"key":"e_1_2_9_45_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaz9356"},{"key":"e_1_2_9_46_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40425-017-0293-7"},{"key":"e_1_2_9_47_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2018-01-828343"},{"key":"e_1_2_9_48_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2017"},{"key":"e_1_2_9_49_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2019-03-900928"},{"key":"e_1_2_9_50_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-16-0597"},{"key":"e_1_2_9_51_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2017.12.001"},{"key":"e_1_2_9_52_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcyt.2017.03.067"},{"key":"e_1_2_9_53_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-01-552174"},{"key":"e_1_2_9_54_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2008-08-175125"},{"key":"e_1_2_9_55_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-3712"},{"key":"e_1_2_9_56_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymthe.2019.04.001"},{"key":"e_1_2_9_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2017.226"},{"key":"e_1_2_9_58_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-18-1811"},{"key":"e_1_2_9_59_1","doi-asserted-by":"publisher","DOI":"10.1136\/jitc-2021-003441"},{"key":"e_1_2_9_60_1","doi-asserted-by":"publisher","DOI":"10.1158\/2643-3230.BCD-21-0084"},{"key":"e_1_2_9_61_1","doi-asserted-by":"publisher","DOI":"10.1002\/jctb.5829"},{"key":"e_1_2_9_62_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-07-1254"},{"key":"e_1_2_9_63_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-10-29"},{"issue":"144","key":"e_1_2_9_64_1","article-title":"In vitro tumor cell rechallenge for predictive evaluation of chimeric antigen receptor T cell antitumor function","author":"Wang D","year":"2019","journal-title":"J vis Exp"}],"container-title":["Clinical &amp; Translational Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cti2.1373","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cti2.1373","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cti2.1373","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,27]],"date-time":"2023-08-27T15:18:31Z","timestamp":1693149511000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cti2.1373"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,1]]},"references-count":63,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2022,1]]}},"alternative-id":["10.1002\/cti2.1373"],"URL":"https:\/\/doi.org\/10.1002\/cti2.1373","archive":["Portico"],"relation":{},"ISSN":["2050-0068","2050-0068"],"issn-type":[{"value":"2050-0068","type":"print"},{"value":"2050-0068","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,1]]},"assertion":[{"value":"2021-07-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-01-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-02-02","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e1373"}}