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Cellular phenotypes from malaria\u2010exposed donors reflected this specialisation for ADCC, with a compartment\u2010wide downregulation of the Fc receptor \u03b3\u2010chain and enrichment of highly differentiated CD56<jats:sup>dim<\/jats:sup> and CD56<jats:sup>neg<\/jats:sup> populations. NK cell degranulation was enhanced in response to opsonised <jats:italic>P.\u2009falciparum<\/jats:italic> schizonts cultured in sickle cell heterozygous erythrocytes relative to wild\u2010type erythrocytes, and when using opsonising plasma collected from donors living in a high transmission area compared to a lower transmission area despite similar levels of 3D7 schizont\u2010specific IgG levels. However, degranulation was lowered in response to opsonised field isolate <jats:italic>P.\u2009falciparum<\/jats:italic> schizonts isolated from clinical malaria infections, compared to the 3D7 laboratory strain typically used in these assays.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This work highlights important host and parasite factors that contribute to ADCC efficacy that should be considered in the design of ADCC assays.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cti2.70005","type":"journal-article","created":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T12:45:02Z","timestamp":1730465102000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Natural killer cell antibody\u2010dependent cellular cytotoxicity to <i>Plasmodium falciparum<\/i> is impacted by cellular phenotypes, erythrocyte polymorphisms, parasite diversity and intensity of 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