{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T04:38:34Z","timestamp":1763008714820,"version":"3.45.0"},"reference-count":24,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T00:00:00Z","timestamp":1761177600000},"content-version":"vor","delay-in-days":295,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["2026122","2026131"],"award-info":[{"award-number":["2026122","2026131"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin &amp; Trans Imm"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      To develop a rapid functional assay to validate variants of uncertain significance (VUS) in the\n                      <jats:italic>MEFV<\/jats:italic>\n                      gene.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Overactivity of the pyrin inflammasome pathway and ASC speck oligomerisation in response to stimulation with low concentrations of\n                      <jats:italic>Clostridium difficile<\/jats:italic>\n                      toxin A was directly visualised by immunofluorescence microscopy. A semi\u2010automated algorithm was developed to count cells and ASC specks.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The semi\u2010automated ASC speck assay is able to discriminate between healthy controls and patients with familial Mediterranean fever (FMF) and pyrin inflammasome overactivity with high sensitivity. It is also able to discriminate pyrin inflammasome overactivity from other autoinflammatory disease controls with high specificity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      The semi\u2010automated ASC speck assay may be a useful test to functionally validate VUS in the\n                      <jats:italic>MEFV<\/jats:italic>\n                      gene and screen for pyrin inflammasome overactivity.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cti2.70054","type":"journal-article","created":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T02:26:44Z","timestamp":1761272804000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["A semi\u2010automated\n                    <scp>ASC<\/scp>\n                    speck assay to evaluate pyrin inflammasome activation"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9022-5101","authenticated-orcid":false,"given":"Pei","family":"Dai","sequence":"first","affiliation":[{"name":"Precision Immunology Program Garvan Institute of Medical Research  Sydney NSW Australia"},{"name":"St Vincent's Healthcare Clinical Campus, School of Clinical Medicine, Faculty of Medicine and Health UNSW Sydney  Sydney NSW Australia"},{"name":"Department of Immunology Nepean Hospital  Sydney NSW Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oliver","family":"Skinner","sequence":"additional","affiliation":[{"name":"Immune Biotherapies Program Garvan Institute of Medical Research  Sydney NSW Australia"},{"name":"Department of Microbiology and Immunology The University of Melbourne at The Peter Doherty Institute for Infection and Immunity  Melbourne VIC Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xufeng","family":"Lin","sequence":"additional","affiliation":[{"name":"Computational Biology Group, Data Science Platform Garvan Institute of Medical Research  Sydney NSW Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aiden","family":"Telfser","sequence":"additional","affiliation":[{"name":"Precision Immunology Program Garvan Institute of Medical Research  Sydney NSW Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie","family":"Ruiz\u2010Diaz","sequence":"additional","affiliation":[{"name":"Precision Immunology Program Garvan Institute of Medical Research  Sydney NSW Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rohit G","family":"Saldanha","sequence":"additional","affiliation":[{"name":"Department of General Paediatrics Sydney Children's Hospital  Sydney NSW Australia"},{"name":"Department of Paediatrics 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