{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T06:25:58Z","timestamp":1772864758898,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,12,10]],"date-time":"2025-12-10T00:00:00Z","timestamp":1765324800000},"content-version":"vor","delay-in-days":343,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010847","name":"Coeliac Australia","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010847","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["APP1176553"],"award-info":[{"award-number":["APP1176553"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["APP2012225"],"award-info":[{"award-number":["APP2012225"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin &amp; Trans Imm"],"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Detection of antigen\u2010specific T\u00a0cells has been crucial for the study of autoimmune illnesses such as coeliac disease (CD). In CD, an oral gluten challenge is required to expand the rare population of circulating gluten\u2010specific T\u00a0cells to detectable levels for IFN\u2010\u03b3 ELISpot detection. We evaluated interleukin (IL)\u20102 detection as a simpler approach to identify gluten\u2010specific T\u00a0cells in CD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      HLA\u2010DQ2.5+\u2010treated CD adults (\n                      <jats:italic>n<\/jats:italic>\n                      \u2009=\u200910) were assessed before and 6\u20138\u2009days after commencing a single\u2010bolus gluten challenge. Serum IL\u20102 (GCIL\u20102), whole blood IL\u20102 (WBA) and peripheral blood mononuclear cell IFN\u2010\u03b3 ELISpot responses were evaluated. Functional effects of peptides encompassing epitopes of varying immunogenicity and pan\u2010HLA\u2010DQ blocking were tested.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      GCIL\u20102 was 90% sensitive in detecting CD. Day 6 post challenge was the optimal day for assessment.\n                      <jats:italic>In vitro<\/jats:italic>\n                      IL\u20102 release showed higher sensitivity than IFN\u2010\u03b3 or IL\u201010 release in the WBA. IL\u20102 WBA had 80% sensitivity prior to gluten challenge, and 90% sensitivity at Day 6 post gluten challenge. IFN\u2010\u03b3 ELISpot was less sensitive: 20% at baseline and 40% at Day 6 after gluten challenge. Pan\u2010HLA\u2010DQ blocking reduced IL\u20102 WBA responses by 89\u201391%, and the assay accurately ranked gluten peptide immunogenicity consistent with published data.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The IL\u20102 WBA provides superior sensitivity over IFN\u2010\u03b3 ELISpot for detecting gluten\u2010specific T\u00a0cells. It can measure the functional blockade of HLA and accurately reflects gluten peptide immunogenicity hierarchies. The IL\u20102 WBA supports immunomonitoring and T\u2010cell epitope mapping in CD and offers broad research and clinical application beyond CD.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cti2.70063","type":"journal-article","created":{"date-parts":[[2025,12,11]],"date-time":"2025-12-11T04:42:55Z","timestamp":1765428175000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["An optimised whole blood interleukin\u20102 release assay is more sensitive than interferon\u2010\u03b3\n                    <scp>ELIS<\/scp>\n                    pot for detecting and quantifying gluten\u2010specific\n                    <scp>CD<\/scp>\n                    4\n                    <sup>+<\/sup>\n                    T\u2010cell responses in coeliac disease"],"prefix":"10.1002","volume":"14","author":[{"given":"Olivia G","family":"Moscatelli","sequence":"first","affiliation":[{"name":"Immunology Division The Walter and Eliza Hall Institute  Parkville VIC Australia"},{"name":"Department of Medical Biology University of Melbourne  Parkville VIC Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amy K","family":"Russell","sequence":"additional","affiliation":[{"name":"Immunology Division The Walter and Eliza Hall Institute  Parkville VIC Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lee M","family":"Henneken","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology The Royal Melbourne Hospital  Parkville VIC Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4923-0678","authenticated-orcid":false,"given":"Melinda Y","family":"Hardy","sequence":"additional","affiliation":[{"name":"Immunology Division The Walter and Eliza Hall Institute  Parkville VIC Australia"},{"name":"Department of Medical Biology University of Melbourne  Parkville VIC 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