{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,6]],"date-time":"2026-11-06T12:32:59Z","timestamp":1793968379349,"version":"4.8.0"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T00:00:00Z","timestamp":1768348800000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin &amp;amp; Trans Imm"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>Anti\u2010dsDNA antibodies are established biomarkers in\u2009systemic lupus erythematosus (SLE), yet the clinical utility of conventional assays remains limited by variable analytical performance. We evaluated a novel Fibre\u2010Optic Surface Plasmon Resonance (FO\u2010SPR) biosensor for real\u2010time profiling of anti\u2010dsDNA antibody\u2013antigen interactions and investigated whether kinetic parameters are associated with disease severity, particularly lupus nephritis (LN).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Sera from 26 SLE patients (including 12 with LN), 14 disease controls and 16 healthy donors were analysed using FO\u2010SPR biosensors coated with biotinylated dsDNA. The sensors generated real\u2010time kinetic profiles from which maximum binding response (\n                      <jats:italic>B<\/jats:italic>\n                      <jats:sub>max<\/jats:sub>\n                      ), association rate and dissociation constant were computed. FO\u2010SPR kinetic results were compared with a conventional chemiluminescent anti\u2010dsDNA assay (CLIA) and correlated with disease activity and renal involvement.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The FO\u2010SPR platform enabled reliable detection of anti\u2010dsDNA antibodies and clearly distinguished SLE patients with renal involvement: Eight of 12 LN patients showed\n                      <jats:italic>B<\/jats:italic>\n                      <jats:sub>max<\/jats:sub>\n                      values above the 90th percentile of the non\u2010SLE control group. In these patients, kinetic parameters revealed high\u2010affinity anti\u2010dsDNA profiles characterised by significantly higher association rates and maximal shifts and lower dissociation constants. The AUC\u2010ROC for SPR\u2010derived affinity parameters was 0.82 (\n                      <jats:italic>P<\/jats:italic>\n                      \u2009=\u20090.006), outperforming the CLIA assay to discriminate lupus patients with versus without nephritis. Furthermore, FO\u2010SPR binding responses correlated with disease activity score in LN.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study highlights the value of an innovative FO\u2010SPR biosensor for real\u2010time characterisation of anti\u2010dsDNA antibody affinity. The kinetic profiles captured by this platform provide clinically relevant insights, particularly for distinguishing LN patients from other SLE phenotypes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cti2.70072","type":"journal-article","created":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T23:44:43Z","timestamp":1768434283000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["A novel surface plasmon resonance approach to assess anti\u2010dsDNA antibody kinetics and disease severity in systemic lupus erythematosus"],"prefix":"10.1002","volume":"15","author":[{"given":"Carole","family":"Nagant","sequence":"first","affiliation":[{"name":"Immunology Department Centre Hospitalier Universitaire Brugmann, Universit\u00e9 libre de Bruxelles  Brussels Belgium"},{"name":"Laboratory of Translational Research, Centre Hospitalier Universitaire Brugmann, Universit\u00e9 libre de Bruxelles  Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1442-9716","authenticated-orcid":false,"given":"Maxime","family":"Taghavi","sequence":"additional","affiliation":[{"name":"Nephrology Department Centre Hospitalier Universitaire Brugmann, Universit\u00e9 libre de Bruxelles  Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-8698-2577","authenticated-orcid":false,"given":"Hasnae Ben Kacem","family":"Ziani","sequence":"additional","affiliation":[{"name":"Laboratory of Translational Research, Centre Hospitalier Universitaire Brugmann, Universit\u00e9 libre de Bruxelles  Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathalie","family":"Ghorra","sequence":"additional","affiliation":[{"name":"Immunology Department Centre Hospitalier Universitaire Brugmann, Universit\u00e9 libre de Bruxelles  Brussels Belgium"},{"name":"Laboratory of Translational Research, Centre Hospitalier Universitaire Brugmann, Universit\u00e9 libre de Bruxelles  Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9rie","family":"Badot","sequence":"additional","affiliation":[{"name":"Rheumatology Department Centre Hospitalier Universitaire Brugmann, Universit\u00e9 libre de Bruxelles  Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Parmentier","sequence":"additional","affiliation":[{"name":"Vixen Bio  Ghent Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francis","family":"Corazza","sequence":"additional","affiliation":[{"name":"Immunology Department Centre Hospitalier Universitaire Brugmann, Universit\u00e9 libre de Bruxelles  Brussels Belgium"},{"name":"Laboratory of Translational Research, Centre Hospitalier Universitaire Brugmann, Universit\u00e9 libre de Bruxelles  Brussels 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