{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T13:51:14Z","timestamp":1781877074082,"version":"3.54.5"},"reference-count":45,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T00:00:00Z","timestamp":1773705600000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clin &amp;amp; Trans Imm"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To develop a therapeutic human papillomavirus (HPV) vaccine capable of intercepting HPV\u2010related cervical disease to reduce the number of new cancer cases and associated deaths.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We previously reported that replication\u2010deficient adenovirus type 26 and 35\u2010based vectors (Ad26, Ad35) expressing a fusion protein of E2, E6 and E7 (E2SH) from HPV16 and HPV18 induced robust HPV\u2010specific T\u2010cell immunity capable of reducing HPV16+ tumors in mice. Here, we assessed immune responses induced by Ad26 and Modified Vaccinia Virus Ankara\u2014strain Bavarian Nordic (MVA\u2010BN) viral vectors expressing E2SH in heterologous vaccination regimens in mice, macaques and a limited number of persistently HPV\u2010infected women (\n                      <jats:italic>N<\/jats:italic>\n                      \u2009=\u20095).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The magnitude and breadth of cellular immune responses were higher in mice that received Ad26.HPV16\/18\u2010MVA\u2010BN.HPV16\/18 than in those that received Ad26.HPV16\/18\u2010Ad35.HPV16\/18, all expressing the fusion protein E2SH of HPV16 and HPV18. Sustained and broad antigen\u2010specific systemic T\u2010cell responses against HPV16 and HPV18 proteins were induced in macaques. In the Phase 1\/2a trial, a 2\u2010dose heterologous immunisation with Ad26.HPV16 or Ad26.HPV18 in Week 0 and MVA\u2010BN.HPV16\/18 in Week 8 induced long\u2010lived antigen\u2010specific cytokine\u2010producing T\u00a0cells. After Week 8, HPV16\/18 infection was no longer detected in 5\/5 active vaccines and 1\/4 placebo recipients during the study period of approximately 1\u2009year. The small number of vaccinated participants did not allow concluding on the relationship between immune responses and viral clearance.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These data show the ability of Ad26.HPV16\/18\u2010MVA\u2010BN.HPV16\/18 heterologous regimens to elicit HPV\u2010specific T\u2010cell responses and warrant further clinical studies to evaluate vaccine efficacy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cti2.70089","type":"journal-article","created":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T05:06:14Z","timestamp":1773810374000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["An Ad26\u2010\n                    <scp>MVA<\/scp>\n                    \u2010\n                    <scp>BN<\/scp>\n                    \u2010based therapeutic vaccine targeting\n                    <scp>HPV16<\/scp>\n                    and\n                    <scp>HPV18<\/scp>\n                    related disease is immunogenic in preclinical models and in women with persistent\n                    <scp>HPV<\/scp>\n                    infections"],"prefix":"10.1002","volume":"15","author":[{"given":"Selina","family":"Khan","sequence":"first","affiliation":[{"name":"Johnson &amp; Johnson  Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satish","family":"Boedhoe","sequence":"additional","affiliation":[{"name":"Johnson &amp; Johnson  Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miranda","family":"Baert","sequence":"additional","affiliation":[{"name":"Johnson &amp; Johnson  Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Serroyen","sequence":"additional","affiliation":[{"name":"Johnson &amp; Johnson  Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thierry","family":"Verbinnen","sequence":"additional","affiliation":[{"name":"Johnson &amp; Johnson  Beerse Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ariane","family":"Volkmann","sequence":"additional","affiliation":[{"name":"Bavarian Nordic GmbH  Martinsried Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Kalla","sequence":"additional","affiliation":[{"name":"Bavarian Nordic GmbH  Martinsried Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrin","family":"Weidner","sequence":"additional","affiliation":[{"name":"Bavarian Nordic GmbH  Martinsried Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathieu Le","family":"Gars","sequence":"additional","affiliation":[{"name":"Johnson &amp; Johnson  Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerry","family":"Sadoff","sequence":"additional","affiliation":[{"name":"Johnson &amp; Johnson  Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michal","family":"Sarnecki","sequence":"additional","affiliation":[{"name":"Johnson &amp; Johnson Janssen Vaccines  Bern Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jort","family":"Vellinga","sequence":"additional","affiliation":[{"name":"Johnson &amp; Johnson  Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jerome","family":"Custers","sequence":"additional","affiliation":[{"name":"Johnson &amp; Johnson  Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gert","family":"Scheper","sequence":"additional","affiliation":[{"name":"Johnson &amp; Johnson  Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanneke","family":"Schuitemaker","sequence":"additional","affiliation":[{"name":"Johnson &amp; Johnson  Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roland","family":"Zahn","sequence":"additional","affiliation":[{"name":"Johnson &amp; Johnson  Leiden The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,3,17]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.27485"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)67069-9"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2214-109X(19)30482-6"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_2_10_6_1","unstructured":"WHO.Cervical Cancer\u2010Fact Sheet. 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