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One such phenotype is the Rh<jats:sub>null<\/jats:sub>, which lacks all the Rh antigens on the red cell membrane and represents one of the rarest blood types in the world with only a few active blood donors available worldwide. Rh<jats:sub>null<\/jats:sub> red cells are critical for the transfusion of immunized patients carrying the same phenotype, besides its utility in the diagnosis of Rh alloimmunization when a high\u2010prevalence Rh specificity is suspected in a patient or a pregnant woman. In both scenarios, the potential use of human\u2010induced pluripotent stem cell (hiPSC)\u2010derived Rh<jats:sub>null<\/jats:sub> red cells is also dependent on ABO compatibility. Here, we present a CRISPR\/Cas9\u2010mediated ABO gene edition strategy for the conversion of blood type A to universal type O, which we have applied to an Rh<jats:sub>null<\/jats:sub> donor\u2010derived hiPSC line, originally carrying blood group A. This work provides a paradigmatic example of an approach potentially applicable to other hiPSC lines derived from rare blood donors not carrying blood type O.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ctm2.1063","type":"journal-article","created":{"date-parts":[[2022,10,25]],"date-time":"2022-10-25T08:40:43Z","timestamp":1666687243000},"source":"Crossref","is-referenced-by-count":12,"title":["ABO gene editing for the conversion of blood type A to universal type O in Rh<sub>null<\/sub> donor\u2010derived human\u2010induced pluripotent stem cells"],"prefix":"10.1002","volume":"12","author":[{"given":"Paolo","family":"Petazzi","sequence":"first","affiliation":[{"name":"Josep Carreras Leukemia Research Institute  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laia","family":"Miquel\u2010Serra","sequence":"additional","affiliation":[{"name":"Immunohematology Laboratory  Barcelona Spain"},{"name":"Transfusional medicine. Vall d'Hebron Research Institute (VHIR)  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sergio","family":"Huertas","sequence":"additional","affiliation":[{"name":"Immunohematology Laboratory  Barcelona Spain"},{"name":"Transfusional medicine. Vall d'Hebron Research Institute (VHIR)  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cecilia","family":"Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Immunohematology Laboratory  Barcelona Spain"},{"name":"Transfusional medicine. Vall d'Hebron Research Institute (VHIR)  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neus","family":"Boto","sequence":"additional","affiliation":[{"name":"Immunohematology Laboratory  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eduardo","family":"Mu\u00f1iz\u2010Diaz","sequence":"additional","affiliation":[{"name":"Immunohematology Laboratory  Barcelona Spain"},{"name":"Transfusional medicine. Vall d'Hebron Research Institute (VHIR)  Barcelona Spain"},{"name":"Department of Medicine Universitat Aut\u00f2noma de Barcelona (UAB)  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo","family":"Men\u00e9ndez","sequence":"additional","affiliation":[{"name":"Josep Carreras Leukemia Research Institute  Barcelona Spain"},{"name":"Department of Biomedicine, School of Medicine University of Barcelona  Barcelona Spain"},{"name":"Centro de Investigaci\u00f3n Biom\u00e9dica en Red de C\u00e1ncer\u2010CIBER\u2010ONC Instituto de Salud Carlos III  Barcelona Spain"},{"name":"Red Espa\u00f1ola de Terapias Avanzadas (TERAV) Instituto de Salud Carlos III (RICORS, RD21\/0017\/0029)"},{"name":"Instituci\u00f3 Catalana de Recerca i Estudis Avan\u00e7ats (ICREA)  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9251-4759","authenticated-orcid":false,"given":"Ana","family":"Sevilla","sequence":"additional","affiliation":[{"name":"Department of Cell Biology Physiology and Immunology, Faculty of Biology, University of Barcelona  Barcelona Spain"},{"name":"Institute of Biomedicine of the University of Barcelona (IBUB)  Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N\u00faria","family":"Nogu\u00e9s","sequence":"additional","affiliation":[{"name":"Immunohematology Laboratory  Barcelona Spain"},{"name":"Transfusional medicine. 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