{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T10:52:27Z","timestamp":1792666347851,"version":"4.5.0"},"reference-count":39,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2022,11,27]],"date-time":"2022-11-27T00:00:00Z","timestamp":1669507200000},"content-version":"vor","delay-in-days":26,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical &amp; Translational Med"],"published-print":{"date-parts":[[2022,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The potential of circulating tumour DNA (ctDNA) as a reliable biomarker for relapse\/metastasis early detection and prognosis in esophageal squamous cell carcinoma (ESCC) after radiotherapy\/chemoradiotherapy (RT\/CRT) initiation requires comprehensive investigation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Treatment\u2010naive locally advanced ESCC patients with available baseline plasma samples were prospectively enrolled from November 2018 to January 2020. RT\/CRT was delivered with a simultaneous integrated boost of radiation dose. Serial plasma samples were collected at baseline (T<jats:sub>0<\/jats:sub>), week 4 of RT\/CRT (T<jats:sub>1<\/jats:sub>), 1\u20103 (T<jats:sub>2<\/jats:sub>) and 3\u20106 months post\u2010RT\/CRT (T<jats:sub>3<\/jats:sub>). ctDNA was analysed using next\u2010generation sequencing of 474 cancer\u2010relevant genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 128 plasma samples from 40 eligible patients were analysed (median age: 64 [range: 40\u201078], 88% males, 95% stage III\/IV), and the median follow\u2010up time was 20.6 months (range: 12.2\u201033.3). During the post\u2010RT\/CRT surveillance including 36 patients, radiological progression was observed in 16 patients, and 69% (11\/16) had detectable post\u2010RT\/CRT ctDNA prior to radiological progression, with a median lead time of 4.4 months compared with radiological imaging. ctDNA positivity at T<jats:sub>1<\/jats:sub> (hazard ratio, HR: 3.60, 95% confidence interval, CI: 1.30\u201010.01) or T<jats:sub>2<\/jats:sub> (HR: 5.45, 95% CI: 1.72\u201017.26) indicated inferior progression\u2010free survival (PFS). ctDNA clearance between T<jats:sub>0<\/jats:sub>\u2010T<jats:sub>1<\/jats:sub> (HR: 0.31, 95% CI: 0.08\u20101.13) or T<jats:sub>0<\/jats:sub>\u2010T<jats:sub>2<\/jats:sub> (HR: 0.11; 95% CI: 0.02\u20100.61) was associated with relatively favourable PFS. Similar results were obtained when focusing on patients without esophagectomy after RT\/CRT. Notably, detectable ctDNA at T<jats:sub>1<\/jats:sub> was a potential indicator of high local recurrence risks (HR: 4.43, 95% CI: 1.31\u201015.04).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>ctDNA was identified as a robust biomarker for early detection of disease progression and post\u2010RT\/CRT prognosis stratification in ESCC. Detectable ctDNA at week 4 of RT\/CRT might indicate higher local recurrence risks, implying the potential clinical utility of ctDNA tests in guiding post\u2010RT\/CRT treatments for locoregional control in ESCC.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ctm2.1116","type":"journal-article","created":{"date-parts":[[2022,11,28]],"date-time":"2022-11-28T05:27:33Z","timestamp":1669613253000},"source":"Crossref","is-referenced-by-count":36,"title":["Prognostic value of circulating tumour DNA during post\u2010radiotherapy surveillance in locally advanced esophageal squamous cell carcinoma"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3149-108X","authenticated-orcid":false,"given":"Xin","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Radiation Oncology, National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nuo","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guowei","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology Cancer Hospital of HuanXing  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianyang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiao","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology, National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaotian","family":"Zhao","sequence":"additional","affiliation":[{"name":"Geneseeq Research Institute Nanjing Geneseeq Technology Inc.  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