{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T19:43:36Z","timestamp":1791315816720,"version":"4.3.1"},"reference-count":44,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2023,7,18]],"date-time":"2023-07-18T00:00:00Z","timestamp":1689638400000},"content-version":"vor","delay-in-days":17,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20211378"],"award-info":[{"award-number":["BK20211378"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical &amp; Translational Med"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The dismal prognosis of hepatocellular carcinoma (HCC) is closely associated with characteristics of the tumour microenvironment (TME). Recent studies have confirmed the presence and potential influence of the microbiome in TME on cancer progression. Elucidating the relationship between microbes in the TME and cancer could provide valuable insights into novel diagnostic markers and therapeutic strategies for HCC and thus warrants a closer investigation of the role of intratumoural microbiome in the HCC TME.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We determined the presence of intratumoural microbiome using fluorescence in situ hybridisation, and explored the microbial community profiles in the HCC TME in paired tumour and adjacent normal tissues using 16S rDNA sequencing. Microbial signatures were characterised in the paired group, and their correlation with clinical characteristics was further investigated. We clustered the microbial signatures of tumour tissues by hepatotypes, and further analysis was performed to elucidate the independent prognostic value of the hepatotypes.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>This study revealed that microbial profiles and community networks differed notably between tumours and adjacent normal tissues. Proteobacteria and Actinobacteria were the most abundant phyla in the HCC TME. The TME microbial profiles also revealed heterogeneities between individuals and between multiple tumour lesions. Clustering of the microbial profiles into two hepatotypes revealed different microbial network patterns. Additionally, the hepatotypes were revealed to be independent prognostic factors in patients with resected HCC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our study illuminates the microbial profiles in the TME of HCC and presents the hepatotype as a potential independent biomarker for the prognostic prediction of HCC after surgery.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ctm2.1331","type":"journal-article","created":{"date-parts":[[2023,7,18]],"date-time":"2023-07-18T10:30:55Z","timestamp":1689676255000},"source":"Crossref","is-referenced-by-count":105,"title":["Intratumoural microbiome can predict the prognosis of hepatocellular carcinoma after surgery"],"prefix":"10.1002","volume":"13","author":[{"given":"Lejia","family":"Sun","sequence":"first","affiliation":[{"name":"Department of Liver Surgery, Peking Union Medical College (PUMC) Hospital PUMC &amp; Chinese Academy of Medical Sciences Beijing China"},{"name":"Department of General Surgery The First Affiliated Hospital of Nanjing Medical University Nanjing China"},{"name":"The First School of Clinical Medicine Nanjing Medical University Nanjing China"},{"name":"Colorectal Institute of Nanjing Medical University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xindi","family":"Ke","sequence":"additional","affiliation":[{"name":"Department of Liver Surgery, Peking Union Medical College (PUMC) Hospital PUMC &amp; Chinese Academy of Medical Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ai","family":"Guan","sequence":"additional","affiliation":[{"name":"Eight\u2010year MD Program Peking Union Medical College (PUMC) &amp; Chinese Academy of Medical Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bao","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Liver Surgery, Peking Union Medical College (PUMC) Hospital PUMC &amp; Chinese Academy of Medical Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiangming","family":"Qu","sequence":"additional","affiliation":[{"name":"Eight\u2010year MD Program Peking Union Medical College (PUMC) &amp; Chinese Academy of Medical Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yinhan","family":"Wang","sequence":"additional","affiliation":[{"name":"Eight\u2010year MD Program Peking Union Medical College (PUMC) &amp; Chinese Academy of Medical Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Xu","sequence":"additional","affiliation":[{"name":"Eight\u2010year MD Program Peking Union Medical College (PUMC) &amp; Chinese Academy of Medical Sciences Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changcan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Liver Surgery, Peking Union Medical College (PUMC) Hospital PUMC &amp; 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