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B cells, particularly those that congregate in tertiary lymphoid structures (TLS), play a significant role in antitumour immunity. However, B\u2010cell heterogeneity at single\u2010cell resolution and its clinical significance with TLS in BC need to be explored further.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Primary tumour lesions and surrounding normal tissues were taken from 14 BC patients, totaling 124,587 cells, for single\u2010cell transcriptome sequencing and bioinformatics analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Based on the usual markers, the single\u2010cell transcriptome profiles were classified into various clusters. A thorough single\u2010cell study was conducted with a focus on tumour\u2010infiltrating B cells (TIL\u2010B) and tumour\u2010associated neutrophils (TAN). TIL\u2010B was divided into five clusters, and unusual cell types, such as follicular B cells, which are strongly related to immunotherapy efficacy, were identified. In BC, TAN and TIL\u2010B infiltration are positively correlated, and at the same time, compared with TLS\u2010high, TAN and TIL\u2010B in TLS\u2010low group are significantly positively correlated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In conclusion, our study highlights the heterogeneity of B cells in BC, explains how B cells and TLS contribute significantly to antitumour immunity at both the single\u2010cell and clinical level, and offers a straightforward marker for TLS called CD23. These results will offer more pertinent information on the applicability and effectiveness of tumour immunotherapy for BC.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ctm2.1346","type":"journal-article","created":{"date-parts":[[2023,8,1]],"date-time":"2023-08-01T07:06:26Z","timestamp":1690873586000},"source":"Crossref","is-referenced-by-count":91,"title":["Single\u2010cell transcriptome sequencing of B\u2010cell heterogeneity and tertiary lymphoid structure predicts breast cancer prognosis and neoadjuvant therapy efficacy"],"prefix":"10.1002","volume":"13","author":[{"given":"Qing","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Breast Surgery Caner Hospital of Yunnan Province The Third Affiliated Hospital of Kunming Medical University Kunming China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Sun","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Primate Biomedical Research Institute of Primate Translational 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