{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T15:16:56Z","timestamp":1767971816771,"version":"3.49.0"},"reference-count":38,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T00:00:00Z","timestamp":1697500800000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical &amp; Translational Med"],"published-print":{"date-parts":[[2023,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Specific microRNAs (miRNAs) were elevated in chronic pancreatitis (CP) patients during islet infusion after total pancreatectomy (TPIAT). We aimed to identify circulating miRNA signatures of pancreatic damage, predict miRNA\u2010mRNA networks to identify potential links to CP pathogenesis and identify islet isolation and transplantation functional outcomes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Small RNA sequencing was performed to identify distinct circulating miRNA signatures in CP. Plasma miRNAs were measured using miRCURY LNA SYBR green quantitative real\u2010time polymerase chain reaction assays. Correlation analyses were performed using R software. The miRNA target and disease interactions were determined using miRNet and the miRNA enrichment and annotation tool.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Alterations were found in circulating miRNAs in CP patients compared to healthy controls. Further studies were conducted on 12 circulating miRNAs enriched in the pancreas, other tissues and other diseases including cancer and fibrosis. Approximately 2888 mRNAs in the pancreas were their targets, demonstrating interactions with 76 small molecules. Three miRNAs exhibited interactions with morphine and five exhibited interactions with glucose. The miRNA panel targeted 22 genes associated with pancreatitis. The islet\u2010specific, acinar cell\u2010specific and liver\u2010specific miRNAs were elevated at 6\u00a0h after islet infusion and returned to baseline levels 3 months after TPIAT. Circulating levels of miRNAs returned to pre\u2010transplant levels 1\u2010year post\u2010transplant. Circulating miRNAs measured before and 6\u00a0h after islet infusion were directly or inversely associated with metabolic outcomes at 3 and 6 months post\u2010transplant.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>miRNAs may contribute to CP pathogenesis, and elevated circulating levels may be specific to pancreatic inflammation and fibrosis, warranting further investigation.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ctm2.1434","type":"journal-article","created":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T07:27:31Z","timestamp":1697527651000},"source":"Crossref","is-referenced-by-count":3,"title":["Clinical and biological significance of circulating miRNAs in chronic pancreatitis patients undergoing total pancreatectomy with islet autotransplantation"],"prefix":"10.1002","volume":"13","author":[{"given":"Srividya","family":"Vasu","sequence":"first","affiliation":[{"name":"Islet Cell Laboratory Baylor Scott and White Research Institute  Dallas Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giovanna","family":"Saracino","sequence":"additional","affiliation":[{"name":"Baylor Simmons Transplant Institute Baylor University Medical Center  Dallas Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carly M.","family":"Darden","sequence":"additional","affiliation":[{"name":"Islet Cell Laboratory Baylor Scott and White Research Institute  Dallas Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenjiro","family":"Kumano","sequence":"additional","affiliation":[{"name":"Department of Gastroenterological Surgery Okayama University Graduate School of Medicine Dentistry and Pharmaceutical Sciences  Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yang","family":"Liu","sequence":"additional","affiliation":[{"name":"The University of Texas Southwestern Medical Center  Dallas Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael C.","family":"Lawrence","sequence":"additional","affiliation":[{"name":"Islet Cell Laboratory Baylor Scott and White Research Institute  Dallas Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0671-9572","authenticated-orcid":false,"given":"Bashoo","family":"Naziruddin","sequence":"additional","affiliation":[{"name":"Baylor Simmons Transplant Institute Baylor University Medical Center  Dallas Texas USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,10,17]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.path.2022.05.005"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2016.02.027"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/mpa.0000000000000713"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pan.2020.04.005"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pan.2018.02.001"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1177\/0963689721999330"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/sla.0000000000001453"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/tp.0000000000000625"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/ajt.14615"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125\u2010019\u20104950\u2010x"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jhbp.377"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/jgh3.12389"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/7293459"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12010197"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep40019"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.54858"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.3109\/00365521.2013.876447"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1097\/md.0000000000006668"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.14806\/ej.17.1.200"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr688"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13059\u2010014\u20100550\u20108"},{"key":"e_1_2_9_23_1","unstructured":"TeamRC The R Project for Statistical Computing. 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