{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,5]],"date-time":"2026-06-05T16:22:26Z","timestamp":1780676546061,"version":"3.54.1"},"reference-count":63,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2024,1,27]],"date-time":"2024-01-27T00:00:00Z","timestamp":1706313600000},"content-version":"vor","delay-in-days":26,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82273017"],"award-info":[{"award-number":["82273017"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical &amp;amp; Translational Med"],"published-print":{"date-parts":[[2024,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Human papillomavirus (HPV) integration into the host genome is an important factor in HPV(+)OPSCC carcinogenesis, in conjunction with HPV oncoproteins E6\/E7. However, a well\u2010studied investigation about virus\u2013host interaction still needs to be completed. Our objective is to characterise HPV integration to investigate potential mechanisms of tumourigenesis independent of E6\/E7 oncoproteins.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>High\u2010throughput viral integration detection was performed on 109 HPV(+)OPSCC tumours with relevant clinicopathological information. Of these tumours, 38 tumours underwent targeted gene sequencing, 29 underwent whole exome sequencing and 26 underwent RNA sequencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>HPV integration was detected in 94% of tumours (with a mean integration count of 337). Tumours occurring at the tonsil\/oropharyngeal wall that exhibit higher PD\u2010L1 expression demonstrated increased integration sites (<jats:italic>p<\/jats:italic>\u00a0=\u00a0.024). HPV exhibited a propensity for integration at genomic sites located within specific fragile sites (FRA19A) or genes associated with functional roles such as cell proliferation and differentiation (PTEN, AR), immune evasion (CD274) and glycoprotein biosynthesis process (FUT8). The viral oncogenes E2, E4, E6 and E7 tended to remain intact. HPV fragments displayed enrichment within host copy number variation (CNV) regions. However, insertions into genes related to altered homologous recombination repair were infrequent. Genes with integration had distinct expression levels. Fifty\u2010nine genes whose expression level was affected by viral integration were identified, for example, EPHB1, which was reported to be involved in cellular protein metabolic process.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>HPV can promote oncogenesis through recurrent integration into functional host genome regions, leading to subsequent genomic aberrations and gene expression disruption. This study characterises viral integrations and virus\u2013host interactions, enhancing our understanding of HPV\u2010related carcinogenesis mechanisms.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ctm2.1556","type":"journal-article","created":{"date-parts":[[2024,1,27]],"date-time":"2024-01-27T11:05:18Z","timestamp":1706353518000},"source":"Crossref","is-referenced-by-count":13,"title":["Mapping the landscape of HPV integration and characterising virus and host genome interactions in HPV\u2010positive oropharyngeal squamous cell carcinoma"],"prefix":"10.1002","volume":"14","author":[{"given":"Shengming","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial\u2010Head Neck Oncology Shanghai Ninth People's Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"College of Stomatology Shanghai Jiao Tong University  Shanghai China"},{"name":"National Center for Stomatology  Shanghai China"},{"name":"National Clinical Research 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