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However, the key genes driving ACC metastasis remain unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Weighted gene co\u2010expression network analysis (WGCNA) and functional enrichment analysis were conducted to identify ACC metastasis\u2010related genes. Data from RNA\u2010seq and microarray were analyzed to reveal correlations of the <jats:italic>CENPM<\/jats:italic> gene with cancer, metastasis, and survival in ACC. Immunohistochemistry was used to assess CENPM protein expression. The impact of CENPM on metastasis behaviour was verified in ACC (H295R and SW\u201013) cells and xenograft NPG mice. DIA quantitative proteomics analysis, western blot, immunofluorescence, and co\u2010immunoprecipitation assay were performed to identify the downstream target of CENPM.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Among the 12\u00a0035 analyzed genes, 363 genes were related to ACC metastasis and <jats:italic>CENPM<\/jats:italic> was identified as the hub gene. CENPM was upregulated in ACC samples and associated with metastasis and poor prognosis. Knockdown of <jats:italic>CENPM<\/jats:italic> inhibited proliferation, invasion, and migration of ACC cells and suppressed liver metastasis in xenograft NPG mice. Collagen\u2010containing extracellular matrix signalling was primarily downregulated when <jats:italic>CENPM<\/jats:italic> was knocked down. FGL1, important components of ECM signalling and immune checkpoint ligand of LAG3, were downregulated following <jats:italic>CENPM<\/jats:italic> silence, overexpressed in human advanced ACC samples, and colocalized with CENPM. Physical interaction between CENPM and FGL1 was identified. Overexpression of <jats:italic>FGL1<\/jats:italic> rescued migration and invasion of <jats:italic>CENPM<\/jats:italic> knockdown ACC cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:italic>CENPM<\/jats:italic> is a key gene in driving ACC metastasis. CENPM promotes ACC metastasis through physical interaction with the immune checkpoint ligand FGL1. CENPM can be used as a new prognostic biomarker and therapeutic target for metastatic ACC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Highlights<\/jats:title><jats:p><jats:list list-type=\"bullet\">\n<jats:list-item><jats:p><jats:italic>CENPM<\/jats:italic> is the key gene that drives ACC metastasis, and a robust biomarker for ACC prognosis.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>Silencing <jats:italic>CENPM<\/jats:italic> impedes ACC metastasis in vitro and in vivo by physical interaction with immune checkpoint ligand FGL1.<\/jats:p><\/jats:list-item>\n<jats:list-item><jats:p>FGL1 is overexpressed in ACC and promotes ACC metastasis.<\/jats:p><\/jats:list-item>\n<\/jats:list><\/jats:p><\/jats:sec>","DOI":"10.1002\/ctm2.70182","type":"journal-article","created":{"date-parts":[[2025,1,8]],"date-time":"2025-01-08T04:20:59Z","timestamp":1736310059000},"source":"Crossref","is-referenced-by-count":7,"title":["Identification of CENPM as a key gene driving adrenocortical carcinoma metastasis via physical interaction with immune checkpoint ligand FGL1"],"prefix":"10.1002","volume":"15","author":[{"given":"Cunru","family":"Zou","sequence":"first","affiliation":[{"name":"Department of Physiology School of Basic Medicine Shandong Second Medical University  Weifang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Physiology School of Basic Medicine Shandong Second Medical University  Weifang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengyue","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Physiology School of Basic Medicine Shandong Second Medical University  Weifang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaxin","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Physiology School of Basic Medicine Shandong Second Medical University  Weifang 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