{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T20:25:29Z","timestamp":1792441529947,"version":"4.5.0"},"reference-count":66,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T00:00:00Z","timestamp":1752019200000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical &amp;amp; Translational Med"],"published-print":{"date-parts":[[2025,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Alzheimer's disease (AD) lacks a less invasive and early detectable biomarker. Here, we investigated the biomarker potential of miR\u2010501\u20103p and miR\u2010502\u20103p using different AD sources.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>MiR\u2010501\u20103p and miR\u2010502\u20103p expressions were evaluated in AD cerebrospinal fluid (CSF) exosomes, serum exosomes, familial and sporadic AD fibroblasts and B\u2010lymphocytes by qRT\u2010PCR analysis. Further, miR\u2010501\u20103p and miR\u2010502\u20103p expressions were analysed in APP, Tau plasmid transfected cells media exosomes and in different brain cell types.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>MiR\u2010501\u20103p and miR\u2010502\u20103p expressions were significantly up\u2010regulated in AD CSF exosomes relative to controls. MiRNA levels were high in accordance with amyloid plaque and NFT density in multiple brain regions. Similarly, both miRNAs were elevated in AD and MCI serum exosomes compared with controls. MiR\u2010502\u20103p expression was high in familial AD and sporadic AD B\u2010lymphocytes. MiR\u2010501\u20103p and miR\u2010502\u20103p expression were elevated intracellularly and secreted extracellularly in response to APP and Tau pathology. Finally, neurons and astrocytes displayed high expression of these miRNAs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Discussion<\/jats:title>\n                    <jats:p>These results suggest that miR\u2010501\u20103p and miR\u2010502\u20103p could be promising biomarkers for AD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key points<\/jats:title>\n                    <jats:p>\n                      <jats:list list-type=\"bullet\">\n                        <jats:list-item>\n                          <jats:p>MiR\u2010501\u20103p and miR\u2010502\u20103p expression is elevated in AD CSF exosomes, AD serum exosomes, AD B\u2010lymphocytes and A\u03b2\u2010 and Tau\u2010treated cells.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>MiR\u2010501\u20103p and miR\u2010502\u20103p are correlated with amyloid plaque and NFT tangle density in specific brain regions.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>MiR\u2010501\u20103p and miR\u2010502\u20103p are highly expressed in neurons and astrocytes, suggesting that these cells are the source of miRNA secretion.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>MiR\u2010501\u20103p and miR\u2010502\u20103p could be a promising biomarker panel for AD<\/jats:p>\n                        <\/jats:list-item>\n                      <\/jats:list>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ctm2.70389","type":"journal-article","created":{"date-parts":[[2025,7,9]],"date-time":"2025-07-09T15:06:49Z","timestamp":1752073609000},"source":"Crossref","is-referenced-by-count":15,"title":["MiRNA\u2010501\u20103p and MiRNA\u2010502\u20103p: A promising biomarker panel for Alzheimer's disease"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4793-9907","authenticated-orcid":false,"given":"Davin","family":"Devara","sequence":"first","affiliation":[{"name":"Center of Emphasis in Neuroscience Department of Molecular and Translational Medicine Paul L. 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Foster School of Medicine Texas Tech University Health Sciences Center El Paso  El Paso Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Rodarte","sequence":"additional","affiliation":[{"name":"Center of Emphasis in Neuroscience Department of Molecular and Translational Medicine Paul L. Foster School of Medicine Texas Tech University Health Sciences Center El Paso  El Paso Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aditi","family":"Kulkarni","sequence":"additional","affiliation":[{"name":"Center of Emphasis in Neuroscience Department of Molecular and Translational Medicine Paul L. Foster School of Medicine Texas Tech University Health Sciences Center El Paso  El Paso Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nathan","family":"Tinu","sequence":"additional","affiliation":[{"name":"Center of Emphasis in Neuroscience Department of Molecular and Translational Medicine Paul L. Foster School of Medicine Texas Tech University Health Sciences Center El Paso  El Paso Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayana","family":"Pai","sequence":"additional","affiliation":[{"name":"Center of Emphasis in Neuroscience Department of Molecular and Translational Medicine Paul L. Foster School of Medicine Texas Tech University Health Sciences Center El Paso  El Paso Texas USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4705-122X","authenticated-orcid":false,"given":"Subodh","family":"Kumar","sequence":"additional","affiliation":[{"name":"Center of Emphasis in Neuroscience Department of Molecular and Translational Medicine Paul L. Foster School of Medicine Texas Tech University Health Sciences Center El Paso  El Paso Texas USA"},{"name":"L. Frederick Francis Graduate School of Biomedical Sciences Texas Tech University Health Sciences Center El Paso  El Paso Texas 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