{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T01:42:36Z","timestamp":1770774156418,"version":"3.50.0"},"reference-count":50,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T00:00:00Z","timestamp":1765929600000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,12,1]],"date-time":"2025-12-01T00:00:00Z","timestamp":1764547200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical &amp;amp; Translational Med"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Paroxysmal nocturnal haemoglobinuria (PNH) is an acquired clonal haematopoietic stem cell disorder. Immune escape is crucial in PNH, and our previous studies revealed that natural killer (NK) cells potential participate in the immune escape of PNH. This study aimed to investigate the subtypes and functional changes of NK cells in PNH patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We analysed CD59\n                      <jats:sup>+<\/jats:sup>\n                      and CD59\n                      <jats:sup>\u2212<\/jats:sup>\n                      bone marrow mononuclear cells using single\u2010cell RNA sequencing (scRNA\u2010seq). The results were validated through flow cytometry and co\u2010culture experiments.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      We classified NK cells into seven subtypes by scRNA\u2010seq, and found significant differences in the distribution of subtypes in CD59\n                      <jats:sup>+<\/jats:sup>\n                      and CD59\n                      <jats:sup>\u2212<\/jats:sup>\n                      NK cell of PNH patients. Compared with controls, the proportion of active and adaptive NK cells was higher in CD59\n                      <jats:sup>+<\/jats:sup>\n                      NK cells. Conversely, the proportion of CD56\n                      <jats:sup>bright<\/jats:sup>\n                      NK cells and terminal NK cells was elevated in CD59\n                      <jats:sup>\u2212<\/jats:sup>\n                      NK cells. Additionally, the proportion of mature NK cells decreased in both the CD59\n                      <jats:sup>+<\/jats:sup>\n                      and CD59\n                      <jats:sup>\u2212<\/jats:sup>\n                      groups. Gene ontology and Kyoto Encyclopedia of Genes and Genomes analysis revealed impaired function of CD59\n                      <jats:sup>\u2212<\/jats:sup>\n                      NK cells, whereas CD59\n                      <jats:sup>+<\/jats:sup>\n                      NK cells showed minimal change. Furthermore, similar results were verified by flow cytometry and co\u2010culture in vivo and in vitro. And the proportion of NK cells was closely related to the proportion of CD8\n                      <jats:sup>+<\/jats:sup>\n                      T cells and the clinical indicators of disease.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      The quantity and function of NK cells in PNH patients are insufficient, in which CD59\n                      <jats:sup>\u2212<\/jats:sup>\n                      NK cells have functional defects, whereas CD59\n                      <jats:sup>+<\/jats:sup>\n                      NK cells were mainly activated and potential involved in immune escape by regulation of T cells.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ctm2.70542","type":"journal-article","created":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T14:54:36Z","timestamp":1765983276000},"source":"Crossref","is-referenced-by-count":0,"title":["Role of NK cells in immune escape in patients with classical paroxysmal nocturnal haemoglobinuria"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6253-4169","authenticated-orcid":false,"given":"Chaomeng","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Hematology Tianjin Medical University General Hospital  Tianjin People's Republic of China"},{"name":"Tianjin Key Laboratory of Bone Marrow Failure and Malignant Hemopoietic Clone Control  Tianjin People's Republic of China"},{"name":"Tianjin Institute of Hematology  Tianjin People's Republic of China"},{"name":"State Key Laboratory of Experimental Hematology  Tianjin People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Hematology Tianjin Medical University General Hospital  Tianjin People's Republic of China"},{"name":"Tianjin Key Laboratory of Bone Marrow Failure and Malignant Hemopoietic Clone Control  Tianjin People's Republic of China"},{"name":"Tianjin Institute of Hematology  Tianjin People's Republic of China"},{"name":"State Key Laboratory of Experimental Hematology  Tianjin People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and Technology ShanghaiTech University  Shanghai People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2732-3646","authenticated-orcid":false,"given":"Liyan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Hematology Tianjin Medical University General Hospital  Tianjin People's Republic of China"},{"name":"Tianjin Key Laboratory of Bone Marrow Failure and Malignant Hemopoietic Clone Control  Tianjin People's Republic of China"},{"name":"Tianjin Institute of Hematology  Tianjin People's Republic of China"},{"name":"State Key Laboratory of Experimental Hematology  Tianjin People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mengting","family":"Che","sequence":"additional","affiliation":[{"name":"Department of Hematology Tianjin Medical University General Hospital  Tianjin People's Republic of China"},{"name":"Tianjin Key Laboratory of Bone Marrow Failure and Malignant Hemopoietic Clone Control  Tianjin People's Republic of China"},{"name":"Tianjin Institute of Hematology  Tianjin People's Republic of China"},{"name":"State Key Laboratory of Experimental Hematology  Tianjin People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7575-0867","authenticated-orcid":false,"given":"Yingying","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Hematology Tianjin Medical University General Hospital  Tianjin People's Republic of China"},{"name":"Tianjin Key Laboratory of Bone Marrow Failure and Malignant Hemopoietic Clone Control  Tianjin People's Republic of China"},{"name":"Tianjin Institute of Hematology  Tianjin People's Republic of China"},{"name":"State Key Laboratory of Experimental Hematology  Tianjin People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Honglei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Hematology Tianjin Medical University General Hospital  Tianjin People's Republic of China"},{"name":"Tianjin Key Laboratory of Bone Marrow Failure and Malignant Hemopoietic Clone Control  Tianjin People's Republic of China"},{"name":"Tianjin Institute of Hematology  Tianjin People's Republic of China"},{"name":"State Key Laboratory of Experimental Hematology  Tianjin People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhaoyun","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Hematology Tianjin Medical University General Hospital  Tianjin People's Republic of China"},{"name":"Tianjin Key Laboratory of Bone Marrow Failure and Malignant Hemopoietic Clone Control  Tianjin People's Republic of China"},{"name":"Tianjin Institute of Hematology  Tianjin People's Republic of China"},{"name":"State Key Laboratory of Experimental Hematology  Tianjin People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lijuan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Hematology Tianjin Medical University General Hospital  Tianjin People's Republic of China"},{"name":"Tianjin Key Laboratory of Bone Marrow Failure and Malignant Hemopoietic Clone Control  Tianjin People's Republic of China"},{"name":"Tianjin Institute of Hematology  Tianjin People's Republic of China"},{"name":"State Key Laboratory of Experimental Hematology  Tianjin People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1969-6015","authenticated-orcid":false,"given":"Hui","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Hematology Tianjin Medical University General Hospital  Tianjin People's Republic of China"},{"name":"Tianjin Key Laboratory of Bone Marrow Failure and Malignant Hemopoietic Clone Control  Tianjin People's Republic of China"},{"name":"Tianjin Institute of Hematology  Tianjin People's Republic of China"},{"name":"State Key Laboratory of Experimental Hematology  Tianjin People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9928-9224","authenticated-orcid":false,"given":"Rong","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Hematology Tianjin Medical University General Hospital  Tianjin People's Republic of China"},{"name":"Tianjin Key Laboratory of Bone Marrow Failure and Malignant Hemopoietic Clone Control  Tianjin People's Republic of China"},{"name":"Tianjin Institute of Hematology  Tianjin People's Republic of China"},{"name":"State Key Laboratory of Experimental Hematology  Tianjin People's Republic of China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,12,17]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrdp.2017.28"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2019003812"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2021012860"},{"issue":"4","key":"e_1_2_11_5_1","first-page":"S20","article-title":"Paroxysmal nocturnal hemoglobinuria: where we stand","volume":"98","author":"Panse J","year":"2023","journal-title":"Am J Hematol"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/bjh.19477"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2007-09-077438"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-020-01238-x"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-023-01737-7"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/08923973.2020.1742733"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-024-01207-0"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.critrevonc.2009.12.001"},{"issue":"16","key":"e_1_2_11_13_1","first-page":"1084","article-title":"Percentages and functions of natural killer cell subsets in peripheral blood of patients with severe aplastic anemia","volume":"91","author":"Li ZS","year":"2011","journal-title":"Zhonghua Yi Xue Za Zhi"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2017.03.003"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.3109\/08820139.2014.888448"},{"issue":"9","key":"e_1_2_11_16_1","first-page":"1221","article-title":"Expression of NK\u2010activating receptor NKp46\/NCR1 on NK cells in patients with severe aplastic anemia","volume":"61","author":"Fu R","year":"2015","journal-title":"Clin Lab"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2025.1511616"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-019-02420-x"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/leu.2010.149"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V92.5.1799"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.1982.tb03931.x"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exphem.2008.09.002"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-03-1337"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/ctm2.1671"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1182\/asheducation-2016.1.208"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-019-0619-0"},{"key":"e_1_2_11_27_1","first-page":"131","article-title":"GseaVis: an R package for enhanced visualization of gene set enrichment analysis in biomedicine","volume":"1","author":"Jun Z","year":"2025","journal-title":"Med Res"},{"key":"e_1_2_11_28_1","first-page":"136","article-title":"Systematic investigation of tumor microenvironment and antitumor immunity with IOBR","volume":"1","author":"Yiran F","year":"2025","journal-title":"Med Res"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.2020009871"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.01.004"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40164-022-00254-5"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.2147\/JBM.S431493"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-06-1669"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2141.2003.04643.x"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI74747"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2141.2011.08914.x"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2014-07-591255"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-06-507574"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2016-08-734285"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2013-06-507574"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2021.108402"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2017.07.004"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2011.02.007"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2021.09.005"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.15537\/smj.2024.45.4.20231010"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41392-021-00728-8"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-09-001222"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-04-273771"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1600815"},{"key":"e_1_2_11_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-023-06156-6"},{"key":"e_1_2_11_51_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.1007022"}],"container-title":["Clinical and Translational Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ctm2.70542","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ctm2.70542","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ctm2.70542","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T21:20:14Z","timestamp":1770758414000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ctm2.70542"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12]]},"references-count":50,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2025,12]]}},"alternative-id":["10.1002\/ctm2.70542"],"URL":"https:\/\/doi.org\/10.1002\/ctm2.70542","archive":["Portico"],"relation":{},"ISSN":["2001-1326","2001-1326"],"issn-type":[{"value":"2001-1326","type":"print"},{"value":"2001-1326","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,12]]},"article-number":"e70542"}}