{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,5]],"date-time":"2026-09-05T04:31:06Z","timestamp":1788582666062,"version":"build-2803163510"},"reference-count":40,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:00:00Z","timestamp":1767830400000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100014137","name":"Shanghai Shenkang Hospital Development Center","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100014137","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical &amp;amp; Translational Med"],"published-print":{"date-parts":[[2026,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Backgroud<\/jats:title>\n                    <jats:p>Persistent infection with high\u2010risk human papillomavirus type 16 (HPV16) is a principal etiological factor in cervical cancer. Nevertheless, the molecular events linking HPV16\u2010associated lesion progression to malignant transformation remain insufficiently characterized, particularly those involving vesicular trafficking and autophagy regulation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Proteomic analysis was conducted across five stages of HPV16\u2010associated cervical lesion progression to identify differentially expressed proteins. The expression of vesicle\u2010associated membrane protein 7 (VAMP7) was validated in cervical tissue specimens and cellular models. Gain\u2010 and loss\u2010of\u2010function approaches were employed to assess the effects of VAMP7 on cellular proliferation, migration, invasion, and apoptosis. Autophagic activity was evaluated by LC3 lipidation, autophagosome accumulation, and analysis of SNARE complexrelated proteins. The in vivo effects of VAMP7 were examined using xenograft tumor models.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>VAMP7 demonstrated dynamic expression changes during cervical lesion progression, characterized by decreased expression in HPV16\u2010positive non\u2010malignant tissues and a gradual increase with disease severity, reaching the highest levels in advanced cervical cancer. Functionally, VAMP7 enhanced proliferation, migration, and invasion while inhibiting apoptosis in cervical cancer cells, whereas distinct effects were observed in non\u2010tumor cervical epithelial cells. Mechanistically, VAMP7 regulated autophagic flux through modulation of SNARE\u2010mediated vesicle fusion, resulting in altered autophagosome accumulation and autophagy\u2010related signaling. In xenograft models, VAMP7 overexpression significantly promoted tumor growth and increased the expression of autophagy\u2010associated markers.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These data indicate that dysregulation of VAMP7\u2010mediated autophagy contributes to cervical carcinogenesis in an HPV16\u2010associated context. VAMP7 may represent a potential therapeutic target for the treatment of cervical cancer.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Key points<\/jats:title>\n                    <jats:p>\n                      <jats:list list-type=\"bullet\">\n                        <jats:list-item>\n                          <jats:p>VAMP7 displays dynamic expression changes during HPV16\u2010associated cervical lesion progression.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>VAMP7 promotes malignant phenotypes of cervical cancer cells by regulating autophagic flux via SNARE\u2010mediated vesicle fusion.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Dysregulated VAMP7\u2010mediated autophagy contributes to cervical carcinogenesis in an HPV16\u2010associated context.<\/jats:p>\n                        <\/jats:list-item>\n                      <\/jats:list>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ctm2.70590","type":"journal-article","created":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T07:20:58Z","timestamp":1767943258000},"source":"Crossref","is-referenced-by-count":1,"title":["VAMP7\u2010mediated autophagy regulates cervical cancer progression associated with persistent HPV16 infection"],"prefix":"10.1002","volume":"16","author":[{"given":"Weijuan","family":"Xin","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Obstetrics and Gynecology Hospital of Fudan University  Shanghai China"},{"name":"Shanghai Key Lab of Reproduction and Development  Shanghai China"},{"name":"Shanghai Key Lab of Female Reproductive Endocrine Related Diseases  Shanghai 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