{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T02:42:12Z","timestamp":1792464132919,"version":"4.5.0"},"reference-count":36,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T00:00:00Z","timestamp":1783209600000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100022963","name":"Key Research and Development Program of Zhejiang Province","doi-asserted-by":"publisher","award":["2025C02054"],"award-info":[{"award-number":["2025C02054"]}],"id":[{"id":"10.13039\/100022963","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82470416"],"award-info":[{"award-number":["82470416"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical &amp; Translational Med"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To determine whether macrophage gasdermin D (GSDMD)\u2010dependent pyroptosis drives acute cardiac allograft rejection and whether targeting GSDMD improves graft survival.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>GSDMD activation was assessed by immunoblotting. Graft survival wasanalysed in global and macrophage\u2010specific GSDMD\u2010deficient recipients. Single\u2010cell RNA\u2010seq identified GSDMD\u2010expressing populations, while immune infiltration and cytokines were measured by flow cytometry and immunohistochemistry; IL1R1 knockout and cytokine challenge were used for validation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      GSDMD deficiency markedly prolonged graft survival. Single\u2010cell profiling showed increased GSDMD expression and pyroptosis primarily in recipient\u2010derived M1 macrophages during acute rejection. Macrophage\u2010specific GSDMD deficiency reduced CD8\n                      <jats:sup>+<\/jats:sup>\n                      T cell\/macrophage infiltration and decreased TNF\u2010\u03b1\u00a0and IL\u20101\u03b2. TNF\u03b1\/IL6 activated NF\u2010\u03baB and JAK\u2013STAT3 to upregulate GSDMD, and IL\u20101\u03b2\u00a0was verified as a key effector for CD8\n                      <jats:sup>+<\/jats:sup>\n                      T cells mediated\u2010rejection. GSDMD pyroptosis inhibitors mitigated rejection and extended graft survival.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Recipient M1 macrophages promote acute rejection via TNF\u03b1\/IL6\u2010driven NF\u2010\u03baB\/STAT3 activation of GSDMD\u2010mediated pyroptosis. Targeting GSDMD represents a promising strategy to enhance cardiac allograft survival.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ctm2.70729","type":"journal-article","created":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T06:44:42Z","timestamp":1783320282000},"source":"Crossref","is-referenced-by-count":3,"title":["Recipient\u2010derived macrophages mediate acute cardiac allograft rejection via GSDMD\u2010induced pyroptosis mechanism"],"prefix":"10.1002","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1286-6181","authenticated-orcid":false,"given":"Bixian","family":"Luo","sequence":"first","affiliation":[{"name":"Department of Gastrointestinal Surgery Fuzhou University Affiliated Provincial Hospital  Fuzhou Fujian China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zelai","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Surgery Second Affiliated Hospital of School of Medicine, Zhejiang University  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