{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T17:32:43Z","timestamp":1787592763323,"version":"build-2736575974"},"reference-count":58,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,7,19]],"date-time":"2026-07-19T00:00:00Z","timestamp":1784419200000},"content-version":"vor","delay-in-days":18,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T00:00:00Z","timestamp":1782864000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2025A1515012725"],"award-info":[{"award-number":["2025A1515012725"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical &amp; Translational Med"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The resolution of inflammation is actively driven by omega\u20103 polyunsaturated fatty acids (PUFAs) via their specialized pro\u2010resolving mediator (SPM) derivatives, including resolvin E1 (RvE1), whose role has been well established. However, clinical application of these mediators is hampered by inherent instability and elevated production costs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      To surmount these obstacles, we have engineered a biosynthetic platform based on the probiotic\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      Nissle 1917 (EcN) that enables controlled, sustained RvE1 production through inducible expression of COX2 and 5\u2010LOX, designated EcN\u2010RvE1. The catalytic capacity, intestinal persistence, and therapeutic efficacy of the platform were evaluated in vitro and in LPS\u2010induced acute inflammation and DSS\u2010induced colitis murine models.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      In this study, we validate the capacity of EcN\u2010RvE1 to catalyse the conversion of eicosapentaenoic acid (EPA) to RvE1 and confirm its ability to achieve long\u2010term intestinal persistence. In murine models of acute inflammation and colitis, EcN\u2010RvE1 exerts marked anti\u2010inflammatory and tissue\u2010protective effects, which are mediated by the regulation of inflammatory cytokine expression and the amelioration of gut microbiota dysbiosis. Moreover, EcN\u2010RvE1 using\n                      <jats:italic>Euglena gracilis<\/jats:italic>\n                      as a photosynthetic protist\u2010based source of PUFAs also exhibits protective anti\u2010inflammatory activity.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Collectively, we report a probiotic engineering platform for the biosynthesis of RvE1, offering a novel strategy for harnessing the anti\u2010inflammatory potential of PUFAs derivatives in clinical settings.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ctm2.70746","type":"journal-article","created":{"date-parts":[[2026,7,20]],"date-time":"2026-07-20T06:57:26Z","timestamp":1784530646000},"source":"Crossref","is-referenced-by-count":1,"title":["Engineered probiotics platform for resolvin E1 biosynthesis confers protection against inflammatory disease"],"prefix":"10.1002","volume":"16","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-1154-3219","authenticated-orcid":false,"given":"Xiaoxiao","family":"Li","sequence":"first","affiliation":[{"name":"Shenzhen Key Laboratory for Systems Medicine in Inflammatory Diseases &amp; Department of Biochemistry Zhongshan School of Medicine Sun Yat\u2010sen University Shenzhen Campus  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiejing","family":"Lin","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory for Systems Medicine in Inflammatory Diseases &amp; Department of Biochemistry Zhongshan School of Medicine Sun Yat\u2010sen University Shenzhen Campus  Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qingqing","family":"Liu","sequence":"additional","affiliation":[{"name":"Shenzhen Key Laboratory for Systems Medicine in Inflammatory Diseases &amp; Department of Biochemistry Zhongshan School of Medicine 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