{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T09:12:01Z","timestamp":1790327521873,"version":"4.1.0"},"reference-count":60,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T00:00:00Z","timestamp":1789084800000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical &amp; Translational Med"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Rationale<\/jats:title>\n                    <jats:p>Circular RNAs (circRNAs) are stable, tissue\u2010 and developmental\u2010stage\u2010specific regulators of gene expression and candidate disease biomarkers. Their expression profile in abnormal lung development in congenital diaphragmatic hernia (CDH) is unknown.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To evaluate circRNA expression profile in CDH\u2010associated abnormal lung development.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We profiled circRNAs in rat CDH and control lungs at embryonic day (E)15 and E21 by microarray. We validated identified circRNAs using back\u2012splice junction amplicon sequencing, RT\u2010qPCR and in situ hybridisation. We modified a circRNA function prediction tool to predict circRNA::micro(mi)RNA::messenger(m)RNA interactions and compared these with Oxford Nanopore RNA sequencing and existing human CDH datasets.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Measurements and main results<\/jats:title>\n                    <jats:p>\n                      Microarrays revealed a unique circRNA biosignature during CDH lung development. CircAnp32e was expressed in a sex\u2010specific and spatiotemporal expression pattern in the epithelium at E15. The predicted mature sequence of circAnp32e overlapped 90% with its human orthologue. circRNA::miRNA::mRNA interaction networks at E15 and E21 revealed enrichment in inflammation\/infection, smooth muscle cell function, cell proliferation\/cell cycle regulation and response to hypoxia pathways. Parental genes of differentially expressed circRNAs at E15 enriched pathways linked to cell proliferation\/cell cycle\/cancer, while at end\u2010gestation, inflammation and cardiovascular processes were also overrepresented. Rat and human CDH lungs showed overlapping pathways with additional enrichment for RNA processing and protein binding\/modification in humans. In a human bronchial epithelial (BEAS\u20102B) nitrofen\u2010injury model,\n                      <jats:italic>ANP32E<\/jats:italic>\n                      and circANP32E were downregulated, and the predicted let\u20107 target was significantly\u00a0dysregulated.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>A unique circRNA signature during abnormal lung development in CDH may mediate inflammatory responses, smooth muscle cell function and cell proliferation regulation via miRNA sponging. Overlap of downstream pathways in rat and human CDH suggests conserved functions across species. This circRNA biosignature defines strong candidate biomarkers for CDH and a basis for future prospective prenatal investigation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ctm2.70752","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T08:43:09Z","timestamp":1789116189000},"source":"Crossref","is-referenced-by-count":0,"title":["Dysregulated circRNA expression profile and associated miRNA sponging in abnormal lung development in congenital diaphragmatic hernia"],"prefix":"10.1002","volume":"16","author":[{"given":"Marietta","family":"Jank","sequence":"first","affiliation":[{"name":"Section of Pediatric Surgery Department of Surgery Max Rady College of Medicine Rady Faculty of Health Sciences University of Manitoba Children's Hospital Research Institute of Manitoba  Winnipeg Manitoba Canada"},{"name":"Department of Pediatric Surgery University Medical Center Mannheim Heidelberg University  Mannheim 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