{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T09:11:58Z","timestamp":1790327518210,"version":"4.1.0"},"reference-count":25,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T00:00:00Z","timestamp":1788220800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7242020"],"award-info":[{"award-number":["7242020"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81402308"],"award-info":[{"award-number":["81402308"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical &amp; Translational Med"],"published-print":{"date-parts":[[2026,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Gastric cancer ovarian metastasis (GCOM) is an aggressive clinical entity whose evolutionary dissemination routes and molecular features remain incompletely defined.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We performed whole\u2010exome sequencing and patient\u2010level phylogenetic reconstruction of 81 spatially distinct tumour specimens from 17 patients with GCOM.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Ovarian metastases showed a higher tumour mutational burden than matched primary tumours and substantial inter\u2010lesional genomic heterogeneity. Phylogenetic reconstruction supported two major evolutionary routes of ovarian dissemination: lymph node\u2010dependent evolution, in which ovarian metastases were phylogenetically associated with lymph node\u2010related lineages, and lymph node\u2010independent evolution, in which ovarian and lymph node metastases diverged along separate branches. Putative driver\u2010gene alterations were relatively enriched in the shared\/truncal mutational fraction, and\n                      <jats:italic>SCAF4<\/jats:italic>\n                      emerged as an understudied candidate metastasis\u2010associated factor. In GC cells,\n                      <jats:italic>SCAF4<\/jats:italic>\n                      depletion was accompanied by widespread alterations in RNA splicing and interferon\u2010related transcriptional programs and enhanced proliferative, migratory, invasive, and clonogenic phenotypes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      These findings support a dual\u2010route evolutionary framework for GCOM and nominate\n                      <jats:italic>SCAF4<\/jats:italic>\n                      as a candidate metastasis\u2010associated factor, providing a genomic basis for understanding the biological heterogeneity of ovarian dissemination in GC.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Highlights<\/jats:title>\n                    <jats:p>\n                      <jats:list list-type=\"bullet\">\n                        <jats:list-item>\n                          <jats:p>A multi\u2010site genomic sequencing and phylogenetic reconstruction in matched gastric primaries and ovarian metastases was performed.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>Uncovered two distinct evolutionary routes for gastric cancer ovarian metastasis: lymph node\u2010dependent and lymph node\u2010independent evolutionary patterns.<\/jats:p>\n                        <\/jats:list-item>\n                        <jats:list-item>\n                          <jats:p>\n                            Genomic and functional analyses nominate\n                            <jats:italic>SCAF4<\/jats:italic>\n                            as a candidate metastasis\u2010associated factor.\n                          <\/jats:p>\n                        <\/jats:list-item>\n                      <\/jats:list>\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ctm2.70847","type":"journal-article","created":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T07:34:31Z","timestamp":1789976071000},"source":"Crossref","is-referenced-by-count":0,"title":["Multi\u2010site sequencing supports dual\u2010track evolution and identifies candidate metastasis\u2010associated factors in gastric cancer ovarian metastasis"],"prefix":"10.1002","volume":"16","author":[{"given":"Peiyu","family":"Zhu","sequence":"first","affiliation":[{"name":"Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education) Gastrointestinal Cancer Center Peking University Cancer Hospital &amp; Institute  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofang","family":"Xing","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Holistic Integrative Management of Gastrointestinal Cancers Beijing Key Laboratory of Carcinogenesis and Translational Research Division of Gastrointestinal Cancer Translational Research Laboratory Peking University Cancer Hospital &amp; Institute  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Ji","sequence":"additional","affiliation":[{"name":"Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education) Gastrointestinal Cancer Center Peking University Cancer Hospital &amp; Institute  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingqian","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education) Gastrointestinal Cancer Center Peking University Cancer Hospital &amp; 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