{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T07:39:35Z","timestamp":1778657975866,"version":"3.51.4"},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,4,12]],"date-time":"2022-04-12T00:00:00Z","timestamp":1649721600000},"content-version":"vor","delay-in-days":11,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical &amp; Translational Med"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:label\/>\n                    <jats:p>SARS\u2010CoV\u20102 specific T\u2010cell response has been associated with disease severity, immune memory and heterologous response to endemic coronaviruses. However, an integrative approach combining a comprehensive analysis of the quality of SARS\u2010CoV\u20102 specific T\u2010cell response with antibody levels in these three scenarios is needed. In the present study, we found that, in acute infection, while mild disease was associated with high T\u2010cell polyfunctionality biased to IL\u20102 production and inversely correlated with anti\u2010S IgG levels, combinations only including IFN\u2010\u03b3 with the absence of perforin production predominated in severe disease. Seven months after infection, both non\u2010hospitalised and previously hospitalised patients presented robust anti\u2010S IgG levels and SARS\u2010CoV\u20102 specific T\u2010cell response. In addition, only previously hospitalised patients showed a T\u2010cell exhaustion profile. Finally, combinations including IL\u20102 in response to S protein of endemic coronaviruses were the ones associated with SARS\u2010CoV\u20102 S\u2010specific T\u2010cell response in pre\u2010COVID\u201019 healthy donors\u2019 samples. These results could have implications for protective immunity against SARS\u2010CoV\u20102 and recurrent COVID\u201019 and may help for the design of new prototypes and boosting vaccine strategies.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ctm2.802","type":"journal-article","created":{"date-parts":[[2022,4,13]],"date-time":"2022-04-13T01:35:55Z","timestamp":1649813755000},"source":"Crossref","is-referenced-by-count":11,"title":["Deciphering the quality of SARS\u2010CoV\u20102 specific T\u2010cell response associated with disease severity, immune memory and heterologous response"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3644-2914","authenticated-orcid":false,"given":"Alberto","family":"P\u00e9rez\u2010G\u00f3mez","sequence":"first","affiliation":[{"name":"Clinical Unit of Infectious Diseases Microbiology and Preventive Medicine Institute of Biomedicine of Seville (IBiS) Virgen del Roc\u00edo University Hospital CSIC University of Seville  Seville Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carmen","family":"Gasca\u2010Capote","sequence":"additional","affiliation":[{"name":"Clinical Unit of Infectious Diseases Microbiology and Preventive Medicine Institute of Biomedicine of Seville (IBiS) Virgen del Roc\u00edo University Hospital CSIC University of Seville  Seville Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5292-1851","authenticated-orcid":false,"given":"Joana","family":"Vitall\u00e9","sequence":"additional","affiliation":[{"name":"Clinical Unit of Infectious Diseases Microbiology and Preventive Medicine Institute of Biomedicine of Seville (IBiS) Virgen del Roc\u00edo University Hospital CSIC University of Seville  Seville Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6583-6974","authenticated-orcid":false,"given":"Francisco J.","family":"Ostos","sequence":"additional","affiliation":[{"name":"Clinical Unit of Infectious Diseases Microbiology and Preventive Medicine Institute of Biomedicine of Seville (IBiS) Virgen del Roc\u00edo University Hospital CSIC University of Seville  Seville Spain"},{"name":"Department of Medical Biochemistry Molecular Biology and Immunology School of Medicine University of Seville  Seville Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Serna\u2010Gallego","sequence":"additional","affiliation":[{"name":"Clinical Unit of Infectious Diseases Microbiology and Preventive Medicine Institute of Biomedicine of Seville (IBiS) Virgen del Roc\u00edo University Hospital CSIC University of Seville  Seville Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda","family":"Trujillo\u2010Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Clinical Unit of Infectious Diseases Microbiology and Preventive Medicine Institute of Biomedicine of Seville (IBiS) Virgen del Roc\u00edo University Hospital CSIC University of Seville  Seville Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Esperanza","family":"Mu\u00f1oz\u2010Muela","sequence":"additional","affiliation":[{"name":"Clinical Unit of Infectious Diseases Microbiology and Preventive Medicine Institute of Biomedicine of Seville (IBiS) Virgen del Roc\u00edo University Hospital CSIC University of Seville  Seville Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teresa","family":"Gir\u00e1ldez\u2010P\u00e9rez","sequence":"additional","affiliation":[{"name":"Clinical Unit of Infectious Diseases Microbiology and Preventive Medicine Institute of Biomedicine of Seville (IBiS) Virgen del Roc\u00edo University Hospital CSIC University of Seville  Seville Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julia","family":"Praena\u2010Segovia","sequence":"additional","affiliation":[{"name":"Clinical Unit of Infectious Diseases Microbiology and Preventive Medicine Institute of Biomedicine of Seville (IBiS) Virgen del Roc\u00edo University Hospital CSIC University of Seville  Seville Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda D.","family":"Navarro\u2010Amuedo","sequence":"additional","affiliation":[{"name":"Clinical Unit of Infectious Diseases Microbiology and Preventive Medicine Institute of Biomedicine of Seville (IBiS) Virgen del Roc\u00edo University Hospital CSIC University of Seville  Seville Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda","family":"Paniagua\u2010Garc\u00eda","sequence":"additional","affiliation":[{"name":"Clinical Unit of Infectious Diseases Microbiology and Preventive Medicine Institute of Biomedicine of Seville (IBiS) Virgen del Roc\u00edo University Hospital CSIC University of Seville  Seville Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuel","family":"Garc\u00eda\u2010Guti\u00e9rrez","sequence":"additional","affiliation":[{"name":"Clinical Unit of Infectious Diseases Microbiology and Preventive Medicine Institute of Biomedicine of Seville (IBiS) Virgen del Roc\u00edo University Hospital CSIC University of Seville  Seville Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuela","family":"Aguilar\u2010Guisado","sequence":"additional","affiliation":[{"name":"Clinical Unit of Infectious Diseases Microbiology and Preventive Medicine Institute of Biomedicine of Seville (IBiS) Virgen del Roc\u00edo University Hospital CSIC University of Seville  Seville Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Inmaculada","family":"Rivas\u2010Jerem\u00edas","sequence":"additional","affiliation":[{"name":"Clinical Unit of Infectious Diseases Microbiology and Preventive Medicine Institute of Biomedicine of Seville (IBiS) Virgen del Roc\u00edo University Hospital CSIC University of Seville  Seville Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mar\u00eda Reyes","family":"Jim\u00e9nez\u2010Le\u00f3n","sequence":"additional","affiliation":[{"name":"Clinical Unit of Infectious Diseases Microbiology and Preventive Medicine Institute of Biomedicine of Seville (IBiS) Virgen del Roc\u00edo University Hospital CSIC University of Seville  Seville Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9000-3787","authenticated-orcid":false,"given":"Sara","family":"Bachiller","sequence":"additional","affiliation":[{"name":"Clinical Unit of Infectious Diseases Microbiology and Preventive Medicine Institute of Biomedicine of Seville (IBiS) Virgen del Roc\u00edo University Hospital CSIC University of Seville  Seville Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto","family":"Fern\u00e1ndez\u2010Villar","sequence":"additional","affiliation":[{"name":"Pneumology Service Galicia Sur Health Research Instituto (IIS Galicia Sur) Complexo Hospitalario Universitario de Vigo  SERGAS\u2010UVigo Vigo Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4836-6768","authenticated-orcid":false,"given":"Alexandre","family":"P\u00e9rez\u2010Gonz\u00e1lez","sequence":"additional","affiliation":[{"name":"Group of Virology and Pathogenesis Galicia Sur Health Research Institute (IIS Galicia Sur) Complexo Hospitalario Universitario de Vigo  SERGAS\u2010UVigo Vigo Spain"},{"name":"Infectious Diseases Unit Department of Internal Medicine Complexo Hospitalario Universitario de Vigo  SERGAS\u2010UVigo Vigo Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3445-1574","authenticated-orcid":false,"given":"Alicia","family":"Guti\u00e9rrez\u2010Valencia","sequence":"additional","affiliation":[{"name":"Clinical Unit of Infectious Diseases Microbiology and Preventive Medicine Institute of Biomedicine of Seville (IBiS) Virgen del Roc\u00edo University Hospital CSIC University of Seville  Seville Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammed","family":"Rafii\u2010El\u2010Idrissi Benhnia","sequence":"additional","affiliation":[{"name":"Clinical Unit of Infectious Diseases Microbiology and Preventive Medicine Institute of Biomedicine of Seville (IBiS) Virgen del Roc\u00edo University Hospital CSIC University of Seville  Seville Spain"},{"name":"Department of Medical Biochemistry Molecular Biology and Immunology School of Medicine University of Seville  Seville Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniela","family":"Weiskopf","sequence":"additional","affiliation":[{"name":"Center for Infectious Disease and Vaccine Research La Jolla Institute for Immunology (LJI)  La Jolla California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro","family":"Sette","sequence":"additional","affiliation":[{"name":"Center for Infectious Disease and Vaccine Research La Jolla Institute for Immunology (LJI)  La Jolla California USA"},{"name":"Department of Medicine Division of Infectious Diseases and Global Public Health University of California  San Diego (UCSD) La Jolla California USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luis F.","family":"L\u00f3pez\u2010Cort\u00e9s","sequence":"additional","affiliation":[{"name":"Clinical Unit of Infectious Diseases Microbiology and Preventive Medicine Institute of Biomedicine of Seville (IBiS) Virgen del Roc\u00edo University Hospital CSIC University of Seville  Seville Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Poveda","sequence":"additional","affiliation":[{"name":"Group of Virology and Pathogenesis Galicia Sur Health Research Institute (IIS Galicia Sur) Complexo Hospitalario Universitario de Vigo  SERGAS\u2010UVigo Vigo Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6747-7813","authenticated-orcid":false,"given":"Ezequiel","family":"Ruiz\u2010Mateos","sequence":"additional","affiliation":[{"name":"Clinical Unit of Infectious Diseases Microbiology and Preventive Medicine Institute of Biomedicine of Seville (IBiS) Virgen del Roc\u00edo University Hospital CSIC University of Seville  Seville Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Virgen del Roc\u00edo Hospital COVID\u201019 and COHVID\u2010GS Working Teams","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,4,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.01.007"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2020.07.005"},{"issue":"1","key":"e_1_2_7_4_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1371\/journal.pone.0245532","article-title":"T cell response to SARS\u2010CoV\u20102 infection in humans: A systematic review","volume":"16","author":"Shrotri M","year":"2021","journal-title":"PLoS One"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.09.038"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.08.017"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2016.05.006"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01505-14"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2598-9"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.202048908"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2021.2019959"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41590-020-0782-6"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2021.109518"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-17488-8"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abf4063"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2550-z"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.05.015"},{"key":"e_1_2_7_18_1","doi-asserted-by":"crossref","unstructured":"MateusJ GrifoniA TarkeA et\u00a0al.Selective and cross\u2010reactive SARS\u2010CoV\u20102 T cell epitopes in unexposed humans.Science.2020;94(October):89\u201094.","DOI":"10.1126\/science.abd3871"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciaa149"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-021-00728-2"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa2007764"},{"issue":"2017","key":"e_1_2_7_22_1","first-page":"54","article-title":"Selective and cross\u2010reactive SARS\u2010CoV\u20102 T cell epitopes in unexposed humans","volume":"3","author":"Mateus J","year":"2020","journal-title":"Science"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.07034-11"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01805-17"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-020-0389-z"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-0913-5"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-73491-5"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0042403"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.181.8.5490"},{"issue":"10","key":"e_1_2_7_30_1","first-page":"1","article-title":"AIM assay","volume":"12","author":"Reiss S","year":"2017","journal-title":"PLoS One."},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1600320"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2020.07.026"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(20)30076-X"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-0901-9"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01281-09"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2020.05.002"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-020-1038-6"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2020.04.027"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2550-z"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.abd2071"}],"container-title":["Clinical and Translational Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ctm2.802","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ctm2.802","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ctm2.802","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,24]],"date-time":"2023-08-24T09:33:24Z","timestamp":1692869604000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ctm2.802"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,4]]},"references-count":39,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2022,4]]}},"alternative-id":["10.1002\/ctm2.802"],"URL":"https:\/\/doi.org\/10.1002\/ctm2.802","archive":["Portico"],"relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2021.12.28.474325","asserted-by":"object"}]},"ISSN":["2001-1326","2001-1326"],"issn-type":[{"value":"2001-1326","type":"print"},{"value":"2001-1326","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,4]]},"article-number":"e802"}}