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An understanding of the relationship between CCC and its carcinogenesis may enable us to prevent and cure GBC. In this study, we attempted to explore changes in the microbiome profile that take place during the transition from chronic cholecystitis mucosa to malignant lesions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Seven paired human GBC and CCC samples were provided by patients who had undergone laparoscopic cholecystectomy or radical cholecystectomy. Mucosal DNA extraction and metagenomic sequencing were performed to evaluate changes in the microbiota between the two groups. We found that GBC patients and CCC patients shared similar stable and permanent dominant species and showed apparent differences in their biliary microbial composition and gene function. <jats:italic>Peptostreptococcus stomatis<\/jats:italic> and <jats:italic>Enterococcus faecium<\/jats:italic> may potentially play a role in GBC progression. In addition, the metagenomic species profiles, co\u2010abundance and co\u2010exclusion correlations, and CAZyme prevalence showed significant differences between the CCC and GBC groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our data suggested that changes in the microbiota between CCC and GBC may help deepen our understanding of the complex spectrum of different microbiotas involved in the development of GBC. Although the cohort size was small, this study has presented the first evidence of the existence of an altered biliary microbiota in GBC, which is clearly different from that in CCC patients.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ctm2.97","type":"journal-article","created":{"date-parts":[[2020,6,11]],"date-time":"2020-06-11T17:04:49Z","timestamp":1591895089000},"source":"Crossref","is-referenced-by-count":33,"title":["A metagenomic study of biliary microbiome change along the cholecystitis\u2010carcinoma sequence"],"prefix":"10.1002","volume":"10","author":[{"given":"Xiaoling","family":"Song","sequence":"first","affiliation":[{"name":"Department of General Surgery and Laboratory of General Surgery, Xinhua Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"Shanghai Key Laboratory of Biliary Tract Disease Research, Xinhua Hospital Shanghai Jiao Tong University School of Medicine  Shanghai China"},{"name":"Shanghai Research Center of Biliary Tract 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