{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T14:02:58Z","timestamp":1789653778541,"version":"4.0.0"},"reference-count":76,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,7,20]],"date-time":"2022-07-20T00:00:00Z","timestamp":1658275200000},"content-version":"vor","delay-in-days":19,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82074159"],"award-info":[{"award-number":["82074159"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81874381"],"award-info":[{"award-number":["81874381"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical &amp; Translational Med"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Cancer\u2010associated fibroblasts (CAFs) contributes to overall tumor progression. In the current survey, we explored the ability of microRNA\u201020a (miR\u201020a) within these CAF\u2010derived exosomes to influence non\u2010small\u2010cell lung cancer (NSCLC) progression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and methods<\/jats:title>\n                    <jats:p>Normal tissue\u2010associated fibroblasts (NAFs) and CAFs were collected from samples of NSCLC patient tumors and paracancerous lung tissues. Exosomes derived from these cells were then characterized via Western blotting, nanoparticle tracking analyses, and transmission electron microscopy. The expression of miR\u201020a was assessed via qPCR and fluorescence in situ hybridization (FISH). CCK\u20108, EdU uptake, and colony formation assessments were used for evaluating tumor proliferation, while Hoechst staining was performed to monitor the in vitro apoptotic death of tumor cells. A model of xenograft tumor established in nude mice was also used to evaluate in vivo tumor responses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>CAF\u2010derived exosomes exhibited miR\u201020a upregulation and promoted NSCLC cell proliferation and resistance to cisplatin (DDP). Mechanistically, CAF\u2010derived exosomes were discovered to transmit miR\u201020a to tumor cells wherein it was able to target PTEN to enhance DDP resistance and proliferation. Associated PTEN downregulation following exosome\u2010derived miR\u201020a treatment enhanced PI3K\/AKT pathway activation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The achieved outcomes explain that CAFs can release miR\u201020a\u2010containing exosomes capable of promoting NSCLC progression and chemoresistance, highlighting this pathway as a possible therapeutic target in NSCLC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/ctm2.989","type":"journal-article","created":{"date-parts":[[2022,7,20]],"date-time":"2022-07-20T14:54:10Z","timestamp":1658328850000},"source":"Crossref","is-referenced-by-count":162,"title":["Cancer\u2010associated fibroblast\u2010derived exosomal microRNA\u201020a suppresses the PTEN\/PI3K\u2010AKT pathway to promote the progression and chemoresistance of non\u2010small cell lung cancer"],"prefix":"10.1002","volume":"12","author":[{"given":"Lin","family":"Shi","sequence":"first","affiliation":[{"name":"Department of Traditional Chinese Medicine Zhujiang Hospital of Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiliang","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Cancer Center Zhujiang Hospital of Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuanyuan","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of VIP Region State Key Laboratory of Oncology in South China Collaborative Innovation Center of Cancer Medicine Sun Yat\u2010sen University Cancer Center  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Zhuo","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine Zhujiang Hospital of Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Siyun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine Zhujiang Hospital of Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaobing","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine Zhujiang Hospital of Southern Medical University  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of VIP Region State Key Laboratory of Oncology in South China Collaborative Innovation Center of Cancer Medicine Sun Yat\u2010sen University Cancer Center  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6242-9898","authenticated-orcid":false,"given":"Bin","family":"Ke","sequence":"additional","affiliation":[{"name":"Department of VIP Region State Key Laboratory of Oncology in South China Collaborative Innovation Center of Cancer Medicine Sun Yat\u2010sen University Cancer Center  Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,7,20]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-24223-1_1"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21590"},{"key":"e_1_2_7_4_1","first-page":"167","article-title":"Molecular diagnostics and targeted therapies in non\u2010small cell lung cancer (NSCLC): an update","volume":"27","author":"Jonna S","year":"2019","journal-title":"Discov Med"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mayocp.2019.01.013"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2015.07.039"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2018.02909"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-18-3962"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/physrev.00048.2019"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2017.12.003"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2018.10.450"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-017-0718-4"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.2967\/jnumed.118.210435"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2019.00356"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2015.07.030"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2019.04.004"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2017.02.020"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.21945"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri3622"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lungcan.2017.12.012"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-019-03233-y"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-biochem-060308-103103"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.31083\/j.jin2004103"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-017-0528-y"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2016.353"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pgen.1006244"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2017.176"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-13-1748"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2022.13269"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgu046"},{"key":"e_1_2_7_31_1","first-page":"22","article-title":"Isolation and characterization of exosomes from cell culture supernatants and biological fluids","volume":"3","author":"Thery C","year":"2006","journal-title":"Curr Protoc Cell Biol"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2020.01.005"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.08.035"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12699"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-017-3132-9"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gni178"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells8111455"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0183435"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkt393"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/75556"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/28.1.27"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0000060"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.211"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-020-1146-4"},{"key":"e_1_2_7_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2017.03.004"},{"key":"e_1_2_7_46_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2016.10.025"},{"key":"e_1_2_7_47_1","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2019.1629865"},{"key":"e_1_2_7_48_1","doi-asserted-by":"publisher","DOI":"10.1159\/000501633"},{"key":"e_1_2_7_49_1","doi-asserted-by":"publisher","DOI":"10.1002\/cac2.12023"},{"key":"e_1_2_7_50_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2010.06.006"},{"key":"e_1_2_7_51_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms11150"},{"key":"e_1_2_7_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2000"},{"key":"e_1_2_7_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/cbin.11000"},{"key":"e_1_2_7_54_1","doi-asserted-by":"publisher","DOI":"10.3390\/antiox9060550"},{"key":"e_1_2_7_55_1","doi-asserted-by":"publisher","DOI":"10.4103\/0973-1482.174185"},{"key":"e_1_2_7_56_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2019.11058"},{"key":"e_1_2_7_57_1","doi-asserted-by":"publisher","DOI":"10.1158\/2326-6066.CIR-17-0640"},{"key":"e_1_2_7_58_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.drup.2019.100644"},{"key":"e_1_2_7_59_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13045-019-0770-1"},{"key":"e_1_2_7_60_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.14346"},{"key":"e_1_2_7_61_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13577-020-00417-8"},{"key":"e_1_2_7_62_1","doi-asserted-by":"publisher","DOI":"10.1158\/1541-7786.MCR-17-0784"},{"key":"e_1_2_7_63_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21165840"},{"key":"e_1_2_7_64_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtn.2019.11.024"},{"key":"e_1_2_7_65_1","doi-asserted-by":"publisher","DOI":"10.2217\/epi-2020-0084"},{"key":"e_1_2_7_66_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gpb.2015.02.001"},{"key":"e_1_2_7_67_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13046-020-01718-4"},{"key":"e_1_2_7_68_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.29111"},{"key":"e_1_2_7_69_1","doi-asserted-by":"publisher","DOI":"10.1159\/000506304"},{"key":"e_1_2_7_70_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-020-03936-y"},{"key":"e_1_2_7_71_1","doi-asserted-by":"publisher","DOI":"10.1080\/20013078.2017.1378056"},{"key":"e_1_2_7_72_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2019.07.009"},{"key":"e_1_2_7_73_1","doi-asserted-by":"publisher","DOI":"10.1002\/JLB.2MIR0817-349R"},{"key":"e_1_2_7_74_1","doi-asserted-by":"publisher","DOI":"10.33549\/physiolres.934345"},{"key":"e_1_2_7_75_1","doi-asserted-by":"publisher","DOI":"10.2217\/pgs.15.122"},{"key":"e_1_2_7_76_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctrv.2013.08.006"},{"key":"e_1_2_7_77_1","doi-asserted-by":"publisher","DOI":"10.1111\/1759-7714.13328"}],"container-title":["Clinical and Translational Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ctm2.989","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/ctm2.989","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ctm2.989","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,23]],"date-time":"2023-08-23T18:05:09Z","timestamp":1692813909000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ctm2.989"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7]]},"references-count":76,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2022,7]]}},"alternative-id":["10.1002\/ctm2.989"],"URL":"https:\/\/doi.org\/10.1002\/ctm2.989","archive":["Portico"],"relation":{},"ISSN":["2001-1326","2001-1326"],"issn-type":[{"value":"2001-1326","type":"print"},{"value":"2001-1326","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7]]},"article-number":"e989"}}