{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T21:17:08Z","timestamp":1790630228427,"version":"4.1.0"},"reference-count":72,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,8,15]],"date-time":"2022-08-15T00:00:00Z","timestamp":1660521600000},"content-version":"vor","delay-in-days":14,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical &amp; Translational Med"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Regulatory T (Treg) cells are important components of the tumour microenvironment (TME) that play roles in gastric cancer (GC) metastasis. Although tumour cells that undergo epithelial\u2010mesenchymal transition (EMT) regulate Treg cell function, their regulatory mechanism in GC remains unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The miR\u2010192\u20105p was identified by examining three Gene Expression Omnibus GC miRNA expression datasets. RNA immunoprecipitation (RIP) and dual\u2010luciferase reporter assays were conducted to identify interactions between miR\u2010192\u20105p and RB1. The role of miR\u2010192\u20105p\/RB1 in GC progression was evaluated based on EdU incorporation, wound healing and Transwell assays. An in vitro co\u2010culture assay was performed to measure the effect of miR\u2010192\u20105p\/RB1 on Treg cell differentiation. In vivo experiments were conducted to explore the role of miR\u2010192\u20105p in GC progression and Treg cell differentiation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>MiR\u2010192\u20105p was overexpressed in tumour and was associated with poor prognosis in GC. MiR\u2010192\u20105p bound to the RB1 3\u2032\u2010untranslated region, resulting in GC EMT, proliferation, migration and invasion. MiR\u2010192\u20105p\/RB1 mediated interleukin\u201010 (IL\u201010) secretion by regulating nuclear factor\u2010kappaBp65 (NF\u2010\u03baBp65), affecting Treg cell differentiation. NF\u2010\u03baBp65, in turn, promoted miR\u2010192\u20105p expression and formed a positive feedback loop. Furthermore, in vivo experiments confirmed that miR\u2010192\u20105p\/RB1 promotes GC growth and Treg cell differentiation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Collectively, our studies indicate that miR\u2010192\u20105p\/RB1 promotes EMT of tumour cells, and the miR\u2010192\u20105p\/RB1\/NF\u2010\u03baBp65 signaling axis induces Treg cell differentiation by regulating IL\u201010 secretion in GC. Our results suggest that targeting miR\u2010192\u20105p\/RB1\/NF\u2010\u03baBp65 \/IL\u201010 may pave the way for the development of new immune treatments for GC.<\/jats:p><\/jats:sec>","DOI":"10.1002\/ctm2.992","type":"journal-article","created":{"date-parts":[[2022,8,15]],"date-time":"2022-08-15T12:06:18Z","timestamp":1660565178000},"source":"Crossref","is-referenced-by-count":45,"title":["MiR\u2010192\u20105p\/RB1\/NF\u2010\u03baBp65 signaling axis promotes IL\u201010 secretion during gastric cancer EMT to induce Treg cell differentiation in the tumour microenvironment"],"prefix":"10.1002","volume":"12","author":[{"given":"Jialin","family":"Song","sequence":"first","affiliation":[{"name":"Department of Gastrointestinal Surgery Zhongnan Hospital of Wuhan University  Wuhan China"},{"name":"Hubei Key Laboratory of Tumour Biological Behaviours  Wuhan China"},{"name":"Hubei Cancer Clinical Study Center  Wuhan 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