{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,10]],"date-time":"2026-07-10T01:41:40Z","timestamp":1783647700283,"version":"3.55.0"},"reference-count":52,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2002,8,21]],"date-time":"2002-08-21T00:00:00Z","timestamp":1029888000000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[2002,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Background: The bcl\u20102 family of proteins are important regulators of apoptosis. Some of the members, such as bcl\u20102 and bcl\u2010x<jats:sub>L<\/jats:sub>, inhibit cell death, whereas others, such as bax and bcl\u2010x<jats:sub>S<\/jats:sub>, promote cell death. We evaluated the ratios of bcl\u20102:bax and bcl\u20102:bcl\u2010x expression by image cytometry in invasive breast carcinoma to determine prognostic significance. Design: Five\u2010micron sections of formalin\u2010fixed, paraffin\u2010embedded tissue from 88 invasive breast carcinomas were immunostained using steam antigen retrieval, an avidin biotin\u2010complex technique with automated stainer and primary antibodies against bcl\u20102 (1\/160; Dako, Carpenteria, CA), bax (1\/1,500; PharMingen, San Diego, CA), and bcl\u2010x (1\/1,500; PharMingen). Positive controls were tonsil (bcl\u20102) and normal breast (bax and bcl\u2010x) tissue samples. Immunostain was measured in 15 high power fields as percentage positive area (PPA) in nuclei and cytoplasm using the CAS 200 image analyzer (Becton Dickinson, San Jose, CA). Results: Median follow\u2010up was 105 months (range 11\u2013130). Significantly improved disease\u2010free survival was found in patients with a bcl\u20102:bcl\u2010x ratio \u2265 1 by univariate and multivariate analyses. The bcl\u20102:bax ratio was not predictive of overall or disease\u2010free survival. A significant difference in overall and disease\u2010free survival was found between carcinomas with positive and negative bcl\u20102 expression by univariate analysis; by multivariate analysis, bcl\u20102 expression was an independent prognostic factor for disease\u2010free survival. The 5\u2010year survival rates were 77% and 50% in patients with bcl\u20102\u2013positive and bcl\u20102\u2013negative carcinomas, respectively. Conclusion: A bcl\u20102:bcl\u2010x ratio \u2265 1, assessed by image cytometry, is significantly associated with improved disease\u2010free survival in patients with invasive breast carcinoma. Significantly increased overall and disease\u2010free survival is associated with positive bcl\u20102 expression. Cytometry (Clin. Cytometry) 50:203\u2013209, 2002. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p>","DOI":"10.1002\/cyto.10091","type":"journal-article","created":{"date-parts":[[2002,8,27]],"date-time":"2002-08-27T01:17:59Z","timestamp":1030411079000},"page":"203-209","source":"Crossref","is-referenced-by-count":8,"title":["Image cytometric bcl\u20102:bax and bcl\u20102:bcl\u2010x ratios in invasive breast carcinoma: Correlation with prognosis"],"prefix":"10.1002","volume":"50","author":[{"given":"A.B.","family":"Schiller","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.S.","family":"Clark","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Cotsonis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Lawson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.B.","family":"DeRose","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Cohen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,12,26]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3874430"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(85)80070-2"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.124.1.1"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.17.7834"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90509-O"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90508-N"},{"key":"e_1_2_6_8_2","first-page":"1323","article-title":"Immunohistochemical 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