{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T02:28:57Z","timestamp":1791167337699,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2002,6,27]],"date-time":"2002-06-27T00:00:00Z","timestamp":1025136000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Cytometry"],"published-print":{"date-parts":[[2002,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Heterogeneity within human dendritic cells (DCs) has been described but its functional relationships to cells of macrophage lineage and its role in human immunodeficiency virus (HIV) infection in vivo remain unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Tonsil macrophages and DCs were isolated from low\u2010density cells by negative selection and DCs were sorted into myeloid and plasmacytoid populations using antibodies to CD11c or CD123. Phagocytosis of latex beads and uptake of dye\u2010labeled target cells were compared by flow cytometry and CD68 and S\u2010100 by immunofluorescence on cytospins of sorted cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Bead uptake and membrane dye transfer were found in both blood and tonsil CD11c<jats:sup>+<\/jats:sup> DCs and in CD14<jats:sup>+<\/jats:sup> cells particularly from blood monocytes. CD11c<jats:sup>\u2010<\/jats:sup> DCs were poorly phagocytic but took up fluorescent dye from intact, necrotic or apoptotic cells. Tonsil DCs and macrophages expressed both CD68 and S\u2010100 but CD11c<jats:sup>\u2010<\/jats:sup> DCs expressed CD68 only.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Freshly isolated CD11c<jats:sup>+<\/jats:sup> tonsil DCs are similar to CD14<jats:sup>+<\/jats:sup> macrophages in phagocytic function but the poorly phagocytic CD11c<jats:sup>\u2010<\/jats:sup> DCs can also take up membrane from target cells. The intracellular markers commonly used to identify DCs and macrophages in situ do not identify accurately the CD11c<jats:sup>\u2010<\/jats:sup> DC subset nor do they distinguish tonsil macrophages from DCs. Cytometry 48:167\u2013176, 2002. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cyto.10118","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T23:33:49Z","timestamp":1030318429000},"page":"167-176","source":"Crossref","is-referenced-by-count":35,"title":["Heterogeneity of freshly isolated human tonsil dendritic cells demonstrated by intracellular markers, phagocytosis, and membrane dye transfer"],"prefix":"10.1002","volume":"48","author":[{"given":"Gianna","family":"Stent","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeanette C.","family":"Reece","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dean 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